4 Host, 96T, apoptosis, array, Assay, Bafilomycin A1, Blocking, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Green, Guinea, Mip 1B, Pamabrom 100Mg, Phospho 4Ebp1, Plant, Plate, Valproic Acid Sodium Salt

EpiGentek – Assay Kits, Antibodies

PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Complete Histone H3 Quantification Package (Colorimetric) is designed for measuring whole histone H3 in plasma or serum. In an assay with this equipment, the histone H3 proteins within the plasma/serum pattern are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade improvement reagent. The ratio of histone H3 is proportional to the depth of absorbance. Absolutely the quantity of H3 might be quantitated by evaluating to the usual management.

What’s DNA Methylation?

DNA methylation happens by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), leading to 5-methylcytosine (5-mC). When a CpG island within the promoter area of a gene is methylated, expression of the gene is repressed. A number of human illnesses akin to most cancers happen when DNA methylation will not be correctly established and/or maintained.

DNA methylation is a dynamic and reversible course of that can lead to a number of intermediate modification varieties. 5-mC transformed from cytosine by DNMTs might be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is additional modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the identical TET enzymes. Evaluation of DNA methylation is crucial to understanding and concentrating on DNA methylation–related adjustments, which might assist determine DNA methylation markers and their organic regulation.

epigentek
epigentek

Which DNA Methylation Device is Proper for Your Experiment?

There are a number of strategies accessible for profiling genome-wide and region-specific DNA methylation. Though value, minimal pattern enter necessities, accuracy, rapidity, and throughput are essential concerns, selecting the best technique is most essential for the success of the evaluation.

Earlier than you start, we advise reviewing any literature that exists associated to your proposed experiment. If different researchers have already accomplished the legwork, you would possibly as nicely reap the benefits of this accessible info as a base to construct your research round. You and your crew can then make use of variations and distinctive angles to make the research totally different and attention-grabbing, reviewing the beforehand accomplished work fastidiously to determine potential obstacles or issues that the earlier researchers encountered.

If you’re not sure which technique will work on your research, we advise beginning with a easy pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the usage of a quick world DNA methylation or hydroxymethylation assay, you’ll be able to assess whether or not your pattern accommodates 5-mC or 5-hmC and the way a lot. Then you’ll be able to decide the subsequent step and which technique is acceptable to make use of. 2) For those who discover your pattern accommodates each 5-mC and 5-hmC, oxidative bisulfite-sequencing may very well be used.

 

This technique can determine and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base decision. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS might be carried out. (3) If the pattern quantity is restricted or single-cell degree evaluation is desired, post-bisulfite sequencing could meet the requirement. (4) For analyzing methylation/hydroxymethylation of particular gene panels akin to a most cancers gene panel or a number of genomic areas, focused bisulfite-sequencing or focused oxidative bisulfite-sequencing will work.

Methylated DNA Quantification

DNA methylation performs a necessary function in regular organismal improvement and mobile differentiation in larger organisms. Gene expression and the event of almost all varieties of most cancers are additionally tied to DNA methylation. For instance, world lower in 5-methylcytosine content material (DNA hypomethylation) is probably going brought on by methyl-deficiency as a result of a wide range of environmental influences and has been proposed as a molecular marker in a number of organic processes akin to most cancers. World quantification of DNA methylation is essential for understanding the roles that gene expression and silencing play in most cancers improvement and different illnesses.

 

BOK Antibody

1-CSB-PA904266
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

BOK Antibody

1-CSB-PA892353LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

BOK Antibody

DF3829 200ul
EUR 304
Description: BOK Antibody detects endogenous levels of total BOK.

BOK Antibody

ABD3829 100 ug
EUR 438

Bok Antibody

3043-100
EUR 359

Bok antibody

20R-1439 100 ug
EUR 651
Description: Rabbit polyclonal Bok antibody

BOK Antibody

3357-002mg 0.02 mg
EUR 171.82
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

BOK Antibody

3357-01mg 0.1 mg
EUR 436.42
Description: BOK Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. BOK, a pro-apoptotic Bcl-2 family member, was initially identified in the ovary, and was found to interact with other Bcl-2 family members such as Mcl-1 and Bfl-1. BOK expression is high during early placental development, suggesting that it may also play a role in regulating trophoblast cell proliferation.

Bok antibody

70R-11577 100 ug
EUR 447
Description: Rabbit polyclonal Bok antibody

BOK Peptide

3357P 0.05 mg
EUR 164.75
Description: (NT) BOK peptide

BOK Peptide

42-325P 0.1 mg
EUR 338
Description: (IN) BOK Peptide

HEK-293T Telomerase Over-Expressing Cell Pellet

abx069991-1Pellet 1 Pellet
EUR 398

Bradford reagent

BDE641 100ml
EUR 61.01

Beaucage reagent

HY-100951 10mM/1mL
EUR 126

BOP reagent

5-02141 25g Ask for price

BOP reagent

5-02142 100g Ask for price

Bluing Reagent

BRT030 30 ml
EUR 60

Bluing Reagent

BRT125 125 ml
EUR 63

Bluing Reagent

BRT3800 1 Gal.
EUR 184

Bluing Reagent

BRT500 500 ml
EUR 76

Bluing Reagent

BRT999 1000 ml
EUR 88

BOP reagent

A7015-100000 100 g
EUR 200
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

BOP reagent

A7015-25000 25 g
EUR 113
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis.

Traut's Reagent

2330-1000
EUR 349

Traut's Reagent

2330-500
EUR 207

MTS Reagent

2808-1000
EUR 990

MTS Reagent

2808-250
EUR 365

MTT Reagent

2809-1G
EUR 180

MTT Reagent

2809-5G
EUR 544

Chymase reagent

30C-CP1129 5 units
EUR 2185
Description: Purified native Human Chymase reagent

BOK Polyclonal Antibody

ES9443-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA