4 Host, 96T, apoptosis, array, Assay, Bafilomycin A1, Blocking, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Green, Guinea, Mip 1B, Pamabrom 100Mg, Phospho 4Ebp1, Plant, Plate, Valproic Acid Sodium Salt

EpiGentek – Assay Kits, Antibodies

PRINCIPLE & PROCEDURE

The EpiQuik  Circulating Complete Histone H3 Quantification Package (Colorimetric) is designed for measuring whole histone H3 in plasma or serum. In an assay with this equipment, the histone H3 proteins within the plasma/serum pattern are captured on the strip wells coated with anti-histone H3 antibody. The captured histone H3 can then be acknowledged with detection antibody adopted by a shade improvement reagent. The ratio of histone H3 is proportional to the depth of absorbance. Absolutely the quantity of H3 might be quantitated by evaluating to the usual management.

What’s DNA Methylation?

DNA methylation happens by the covalent addition of a methyl group (CH3) on the 5-carbon of the cytosine ring by DNA methyltransferases (DNMTs), leading to 5-methylcytosine (5-mC). When a CpG island within the promoter area of a gene is methylated, expression of the gene is repressed. A number of human illnesses akin to most cancers happen when DNA methylation will not be correctly established and/or maintained.

DNA methylation is a dynamic and reversible course of that can lead to a number of intermediate modification varieties. 5-mC transformed from cytosine by DNMTs might be hydroxylated to 5-hydroxymethylcytosine (5-hmC) by TET enzymes. 5-hmC is additional modified to 5-formylcytosine (5-fC) and 5-carboxycytosine (5-caC) by the identical TET enzymes. Evaluation of DNA methylation is crucial to understanding and concentrating on DNA methylation–related adjustments, which might assist determine DNA methylation markers and their organic regulation.

epigentek
epigentek

Which DNA Methylation Device is Proper for Your Experiment?

There are a number of strategies accessible for profiling genome-wide and region-specific DNA methylation. Though value, minimal pattern enter necessities, accuracy, rapidity, and throughput are essential concerns, selecting the best technique is most essential for the success of the evaluation.

Earlier than you start, we advise reviewing any literature that exists associated to your proposed experiment. If different researchers have already accomplished the legwork, you would possibly as nicely reap the benefits of this accessible info as a base to construct your research round. You and your crew can then make use of variations and distinctive angles to make the research totally different and attention-grabbing, reviewing the beforehand accomplished work fastidiously to determine potential obstacles or issues that the earlier researchers encountered.

If you’re not sure which technique will work on your research, we advise beginning with a easy pre-screen of worldwide DNA methylation and hydroxymethylation standing in your samples. 1) With the usage of a quick world DNA methylation or hydroxymethylation assay, you’ll be able to assess whether or not your pattern accommodates 5-mC or 5-hmC and the way a lot. Then you’ll be able to decide the subsequent step and which technique is acceptable to make use of. 2) For those who discover your pattern accommodates each 5-mC and 5-hmC, oxidative bisulfite-sequencing may very well be used.

 

This technique can determine and distinguish 5-mC from 5-hmC on a genome-wide scale at single-base decision. If solely 5-mC is detected with little or no 5-hmC, typical WGBS or RRBS might be carried out. (3) If the pattern quantity is restricted or single-cell degree evaluation is desired, post-bisulfite sequencing could meet the requirement. (4) For analyzing methylation/hydroxymethylation of particular gene panels akin to a most cancers gene panel or a number of genomic areas, focused bisulfite-sequencing or focused oxidative bisulfite-sequencing will work.

Methylated DNA Quantification

DNA methylation performs a necessary function in regular organismal improvement and mobile differentiation in larger organisms. Gene expression and the event of almost all varieties of most cancers are additionally tied to DNA methylation. For instance, world lower in 5-methylcytosine content material (DNA hypomethylation) is probably going brought on by methyl-deficiency as a result of a wide range of environmental influences and has been proposed as a molecular marker in a number of organic processes akin to most cancers. World quantification of DNA methylation is essential for understanding the roles that gene expression and silencing play in most cancers improvement and different illnesses.

 

Bok antibody

20R-1439 100 ug
EUR 651
Description: Rabbit polyclonal Bok antibody

Bok antibody

70R-11577 100 ug
EUR 447
Description: Rabbit polyclonal Bok antibody

BOK Antibody

1-CSB-PA892353LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
  • Form: Liquid
  • Buffer: Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 >95%, Protein G purified
Description: A polyclonal antibody against BOK. Recognizes BOK from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200

BOK Antibody

1-CSB-PA904266
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:25-1:100

BOK Antibody

DF3829 200ul
EUR 304
Description: BOK Antibody detects endogenous levels of total BOK.

BOK Antibody

1-CSB-PA437965
  • EUR 317.00
  • EUR 244.00
  • 100ul
  • 50ul
  • Form: Liquid
  • Buffer: -20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol Antigen affinity purification
Description: A polyclonal antibody against BOK. Recognizes BOK from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:2000-1:5000, IHC:1:20-1:100

BOK siRNA

20-abx900655
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

BOK siRNA

20-abx909215
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

BOK siRNA

20-abx909216
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol
  • Shipped within 5-10 working days.

BOK Antibody

ABD3829 100 ug
EUR 438

HEK-293T Telomerase Over-Expressing Cell Pellet

abx069991-1Pellet 1 Pellet
EUR 398
  • Shipped within 1-3 working days.

BOK Polyclonal Antibody

29816-100ul 100ul
EUR 252

BOK Polyclonal Antibody

29816-50ul 50ul
EUR 187

BOK Rabbit pAb

A16354-100ul 100 ul
EUR 308

BOK Rabbit pAb

A16354-200ul 200 ul
EUR 459

BOK Rabbit pAb

A16354-20ul 20 ul
EUR 183

BOK Rabbit pAb

A16354-50ul 50 ul
EUR 223

Polyclonal Bok Antibody

APR00010G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Bok . This antibody is tested and proven to work in the following applications:

BOK Blocking Peptide

DF3829-BP 1mg
EUR 195

Polyclonal BOK Antibody

APR06350G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human BOK . This antibody is tested and proven to work in the following applications:

BOK Polyclonal Antibody

ABP57917-003ml 0.03ml
EUR 158
  • Immunogen information: Synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Polyclonal Antibody

ABP57917-01ml 0.1ml
EUR 289
  • Immunogen information: Synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Polyclonal Antibody

ABP57917-02ml 0.2ml
EUR 414
  • Immunogen information: Synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90
  • Applications tips:
Description: A polyclonal antibody for detection of BOK from Human, Mouse, Rat. This BOK antibody is for WB, ELISA. It is affinity-purified from rabbit serum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from part region of human BOK protein at amino acid sequence of 10-90

BOK Polyclonal Antibody

ES9443-100ul 100ul
EUR 279
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

BOK Polyclonal Antibody

ES9443-50ul 50ul
EUR 207
Description: A Rabbit Polyclonal antibody against BOK from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA

Anti-BOK antibody

STJ118794 100 µl
EUR 277

Anti-BOK antibody

STJ72368 100 µg
EUR 359

Anti-BOK antibody

STJ190601 200 µl
EUR 197
Description: Unconjugated Rabbit polyclonal to BOK

Chymase reagent

30C-CP1129 5 units
EUR 2185
Description: Purified native Human Chymase reagent

Traut's Reagent

2330-1000
EUR 349

Traut's Reagent

2330-500
EUR 207