EVALUATION OF EPIANDROSTERONE AS A LONG-TERM MARKER OF TESTOSTERONE USE
Identification and analysis of long-term markers is essential in prolonging the detection window for anabolic steroid abuse in sport. Just lately, sulfoconjugated epiandrosterone was recognized as a possible long-term marker for the abuse of sure endogenous anabolic brokers, together with testosterone, which continues to be broadly used as a efficiency enhancing agent in sport. To guage the applicability of epiandrosterone sulfate as a marker for testosterone use, administration research have been performed with a number of modes of testosterone administration – transdermal, intramuscular, and subcutaneous. A modified pattern preparation methodology was used to gather each glucuronidated and sulfoconjugated analytes of curiosity. Carbon isotope ratio measurements from the administration research are introduced right here.
Epiandrosterone was much less efficient than the conventionally used goal compounds for detection of the low dose utility (transdermal gel). With intramuscular administration, epiandrosterone was extra diagnostic than with transdermal administration, but it surely didn’t extend the detection window greater than the traditional goal compounds.
With subcutaneous administration, the doses administered to the topics have been diversified and the impact within the epiandrosterone values was depending on the magnitude of the dose administered. Epiandrosterone doesn’t seem like a helpful marker within the detection of low dose testosterone administration. It’s aware of greater dose administration, but it surely doesn’t present extension of the detection window relative to traditional goal compounds.
Growth and validation of a quick gasoline chromatography combustion isotope ratio mass spectrometry methodology for the detection of epiandrosterone sulfate in urine.
In doping management, to substantiate the exogenous origin of exogenously administered anabolic androgenic steroids (AAS), a gasoline chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS) evaluation is carried out. Just lately printed work suggests epiandrosterone sulfate (EpiAS) as a promising IRMS goal compound for the detection of AAS, able to prolonging the detection window. Nevertheless, EpiAS is barely excreted in urine in its sulfoconjugated kind whereas all different IRMS goal compounds are excreted glucuronidated, that means that EpiAS can’t be included within the present IRMS strategies. A separate in depth pattern preparation must be carried out on this compound with a special hydrolysis and extraction process and a special liquid chromatography (LC) clean-up.
The present work presents a brand new, quick and simple to implement EpiAS IRMS methodology. The method was based mostly on the direct GC evaluation of non-hydrolysed EpiAS, making the strong section extraction, hydrolysis and acetylation step redundant. Pattern preparation consisted of a easy liquid liquid extraction, adopted by LC fraction assortment. A inhabitants research was carried out to examine the compliance with the factors drafted by the World Anti-Doping Company (WADA). To confirm the applicability of the developed method, the strategy was utilized to the samples of 4 administration research (i.e., dehydroepiandrosterone (DHEA), testosterone gel (T gel), androstenedione (ADION) and intramuscular testosterone undecanoate. In distinction to beforehand printed information, the energy of EpiAS as goal compound and the prolongation of the detection window as compared with the traditional IRMS goal compounds was much less pronounced.