Epigentek Chromoplasts Chromatin Extraction Kit

Background Information

Chromatin immunoprecipitaton (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, the ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly demanded.

Product Overview

The ChromaFlash Chromatin Extraction Package deal is a complete set of optimized buffers and reagents for isolating chromatin or DNA-protein superior from mammalian cells or tissues in a straightforward and quick format. Chromatin prepared by this bundle could be utilized in various chromatin immunoprecipitation methods. Moreover it’s the actually helpful methodology for buying chromatin required by Epigentek’s one-hour ChIP methodology using the ChromaFlash One-Step ChIP Package deal. The isolated chromatin may also be utilized in several chromatin-related features equal to in vitro protein-DNA binding assays and nuclear enzyme assays.

  • Terribly fast course of: your complete course of from cell/tissue sample to ready-to-use chromatin is decrease than 60 minutes.
  • Helpful and versatile: the bundle is acceptable for preparing every native chromatin and cross-linked chromatin from monolayer or suspension cells or tissues.
  • Unsheared chromatin makes it customizable for quite a few analysis workflows that require each intact or fragmented chromatin, along with ChIP, in vitro protein-DNA interaction analysis, nuclear enzyme assay, and so forth.
epigentek chromoplasts chromatin
epigentek chromoplasts chromatin

Starting Provides & Enter Amount
Starting provides can embrace quite a few tissue or cell samples equal to cells from flask or microplate cultured cells, up to date and frozen tissues, and so forth. The amount of cells and tissues for each preparation might be 1 x 105 to 5 x 106 cells and 10 mg to 200 mg, respectively. For optimum preparation, the enter amount should be 1 to 5 x 106 cells or 50 to 200 mg tissues.  Yield of chromatin is roughly 4 ug per 106  cells or per 50 mg tissues.

Background Information
Chromatin immunoprecipitation (ChIP) offers an advantageous gadget for locating out protein-DNA interaction. With ChIP, the experimenter can resolve if a specific protein binds to the exact sequences of a gene in residing cells by combining it with PCR (ChIP-PCR), microarray (ChIP-chip), or sequencing (ChIP-Seq) strategies. For example, the measurement of the amount of methylated histone H3 at lysine 9 (meH3-K9) associated to a specific gene promoter space beneath quite a few conditions might be achieved by way of a ChIP-PCR assay, whereas recruitment of meH3-K9 to the promoters on a genome-wide scale might be detected by ChIP-chip. Notably, a ChIP methodology with specific antibodies straight in direction of quite a few transcriptional parts is broadly in demand.

Principle & Course of
The ChromaFlash™ Plant Chromatin Extraction Package deal accommodates the entire reagents required for ending up a worthwhile chromatin extraction straight from vegetation. Cell membranes of the sample, with or with out cross-linking, are broken down using the equipped lysis buffer. Chromatin or DNA-protein superior is then extracted with the extraction buffer. The extracted chromatin can then be diluted with chromatin buffer and saved on the relevant temperature.

ExoDNA? Extraction Kit

K1242-50
EUR 881

miRNA Extraction Kit

K1456-50 50 Preps
EUR 551

Membrane Protein Extraction Kit

55R-1357 50 assays
EUR 740
Description: Membrane Protein Extraction Kit for use in the research laboratory

Mammalian Cell Extraction Kit

55R-1358 500 assays
EUR 381
Description: Mammalian Cell Extraction Kit for use in the research laboratory

Quick Gel Extraction Kit

20-abx098085
  • EUR 286.00
  • EUR 203.00
  • 200 rxns
  • 50 rxns
  • Shipped within 5-10 working days.

Up RNA Extraction Kit

20-abx098087
  • EUR 258.00
  • EUR 105.00
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.

Plant RNA extraction Kit

20-abx098088
  • EUR 258.00
  • EUR 105.00
  • 100 ml
  • 3 ml
  • Shipped within 5-10 working days.

Genomic DNA Extraction Kit

K5016005 1 kit
EUR 329

Membrane Protein Extraction Kit

K268-50
EUR 539

Mammalian Cell Extraction Kit

K269-500
EUR 294

Total RNA Extraction Kit

K2014005 1 kit
EUR 344
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Plant Tissue Extraction Kit

K296-50
EUR 457

Total Protein Extraction Kit

K3011010 1 kit
EUR 370
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit

K3014005 1 kit
EUR 382
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein Extraction Kit

K2113-50 50 assays
EUR 544

Mammalian Cell Extraction Kit

K2114-500 500 assays
EUR 321

Exosomal RNA Extraction Kit

P200R,-part-of-P200 NULL
EUR 0

Exosomal DNA Extraction Kit

P230-25 NULL
EUR 0

Exosomal DNA Extraction Kit

P230-50 NULL
EUR 0

EpiQuik Nuclear Extraction Kit

OP-0002 100 Assays
EUR 292.15
Description: kits suitable for this type of research

Bacterial RNA Extraction Kit

R403-01 100 rxn
EUR 155

Total Protein Extraction Kit

BSP003 50Preps
EUR 109.16
  • Product category: Molecular Biology Kits/Protein - Extraction/Animal