Proteinase Okay – one in every of many sometimes used enzymes in molecular biology.
Superb totally different for protein digestion in assorted options.
BLIRT – expert in Proteinase Okay manufacturing for over 15 years.
Licensed manufacturing course of ISO 13485 ensuing all through the very best high quality product with a really low batch-to-batch variability.
Big elements, the possibility of white labeling, and portioning service – liquid selection all through the quantity on the consumer’s request (as rather a lot as quite a lot of million samples month-to-month).
Thermo Scientific Proteinase Okay is a broad-range endolytic protease extensively used for digestion of proteins in nucleic acid preparations. It degrades proteins even all through the presence of detergents. Proteinase Okay cleaves peptide bonds on the carboxylic sides of aliphatic, fragrant, or hydrophobic amino acids. The Proteinase Okay is classed as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.
Proteinase Okay (Lyophilized)
For Basic Digestion of Protein in Pure Samples
Lively over a pH differ of 4.3–12.0, in 0.5% SDS or 1% Triton® X-100
Purified to take away RNase and DNase actions
Secure at Room Temperature and Straightforward to Use
Lively over pH differ 4.3–12.Zero in 0.5% SDS or 1% Triton X-100
Retains >80% prepare at temperatures as rather a lot as 60°C
No resuspension or thawing ahead of use
Cat.# MC5005, MC5008 provided at a highlight of 20mg/ml
Proteinase K, recombinant
Highlights
• Prepared-to-use reply • Lively in a variety of response circumstances
Capabilities
• Isolation of genomic DNA from mouse tail • Isolation of genomic DNA from cultured cells • Elimination of DNases and RNases when isolating DNA and RNA from tissues or cell traces • Dedication of enzyme localization • Enhancing cloning effectivity of PCR merchandise
Keep in mind
• The really useful working focus of Proteinase Okay is 0.05 to 1 mg/mL. The prepare of the enzyme is stimulated by 0.2 to 1% SDS or by 1 to Four M urea • Ca2+ protects Proteinase Okay in route of autolysis, will enhance the thermal stability, and has a regulatory perform for the substrate binding web site of Proteinase Okay • Secure over an enormous pH differ: 4.Zero to 12.5, optimum pH 7.5 to eight.0 • Optimum prepare at 50 to55°C • Speedy denaturation of enzyme happens at temperatures above 65°C.
Proteinase Okay Molecular Biology Grade from Parengyodontium album (Tritirachium album) is a subtilisin-related serine protease. It’s a broad-spectrum endopeptidase with a really excessive particular prepare.
Recombinant Proteinase Okay enzyme is expressed in Pichia pastoris, and undergoes in depth purification to yield the best high quality product.
Proteinase Okay Molecular Biology Grade (PCR Grade) is filled with life beneath a variety of response circumstances, together with elevated temperatures and the presence of SDS. In consequence, this enzyme is extensively utilizing for the digestion of proteins, together with DNases and RNases, all by nucleic acid preparations with out compromising the integrity of remoted DNA or RNA.
Decisions
Recombinant broad-spectrum non-specific protease derived from Tritirachium album and over-expressed in Pichia pastoris.
Excessive prepare and distinctive purity.
Lively at excessive temperatures (as rather a lot as 56 °C) and denaturing circumstances (e.g. all through the presence of urea and/or SDS), which makes it best for digesting proteins in quite a lot of options.
Secure over an enormous pH differ: 4.0–12.5 (optimum pH 7.5–8.0).
Decreased quantity of host DNA (≤ 10 pg/mg / MBG or ≤ 0.1 pg/mg NGS).
Accessible as powder, lyophilized “cake” or liquid.
Capabilities:
Extraction of DNA and RNA from fully completely totally different beginning offers.
Purification of goal provides from contaminating proteins.
Elimination of DNases and RNases all by nucleic acids isolation.
Description: Proteinase K is a serine protease often used to digest protein and to remove contamination in nucleic acid preparations. It does so by inactivating nucleases which could otherwise cause the break down of DNA and/or RNA.
Description: Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K can be use for promoting methods of viral nucleic acid extraction, and detection[1][2][3][4].
