4 Host, 96T, apoptosis, array, Bacteria Pig Pigeon, Bafilomycin A1, Blocking, Ch 223191, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Goat, Green, Mip 1B, Pamabrom 100Mg, Pepstatin A, Phospho 4Ebp1, Plant, plasmid, Plate, Tubastatin A, Valproic Acid Sodium Salt

Mass Measurements of Neutron-Deficient Yb Isotopes and Nuclear Structure at the Extreme Proton-Rich Side of the N=82 Shell

High-accuracy mass measurements of neutron-deficient Yb isotopes have been performed at TRIUMF using TITAN’s multiple-reflection time-of-flight mass spectrometer (MR-TOF-MS). For the first time, an MR-TOF-MS was used on line simultaneously as an isobar separator and as a mass spectrometer, extending the measurements to two isotopes further away from stability than otherwise possible.
The ground state masses of ^{150,153}Yb and the excitation energy of ^{151}Yb^{m} were measured for the first time. As a result, the persistence of the N=82 shell with almost unmodified shell gap energies is established up to the proton drip line. Furthermore, the puzzling systematics of the h_{11/2}-excited isomeric states of the N=81 isotones are unraveled using state-of-the-art mean field calculations.

The CERN-MEDICIS Isotope Separator Beamline

CERN-MEDICIS is an off-line isotope separator facility for the extraction of radioisotopes from irradiated targets of interest to medical applications. The beamline, between the ion source and the collection chamber, consists of ion extraction and focusing elements, and a dipole magnet mass spectrometer recovered from the LISOL facility in Louvain-la-Neuve.
The latter has been modified for compatibility with MEDICIS, including the installation of a window for injecting laser light into the ion source for resonance photo-ionization. Ion beam optics and magnetic field modeling using SIMION and OPERA respectively were performed for the design and characterization of the beamline. The individual components and their optimal configuration in terms of ion beam extraction, mass separation, and ion transport efficiency is described, along with details of the commissioning and initial performance assessment with stable ion beams.

Triple Oxygen Isotope Measurements (Δ’ 17 O) of Body Water Reflect Water Intake, Metabolism, and δ 18 O of Ingested Water in Passerines

Understanding physiological traits and ecological conditions that influence a species reliance on metabolic water is critical to creating accurate physiological models that can assess their ability to adapt to environmental perturbations (e.g., drought) that impact water availability. However, relatively few studies have examined variation in the sources of water animals use to maintain water balance, and even fewer have focused on the role of metabolic water. A key reason is methodological limitations.
Here, we applied a new method that measures the triple oxygen isotopic composition of a single blood sample to estimate the contribution of metabolic water to the body water pool of three passerine species. This approach relies on Δ’17O, defined as the residual from the tight linear correlation that naturally exists between δ17O and δ18O values. Importantly, Δ’17O is relatively insensitive to key fractionation processes, such as Rayleigh distillation in the water cycle that have hindered previous isotope-based assessments of animal water balance.
  • We evaluated the effects of changes in metabolic rate and water intake on Δ’17O values of captive rufous-collared sparrows (Zonotrichia capensis) and two invertivorous passerine species in the genus Cinclodes from the field.
  • As predicted, colder acclimation temperatures induced increases in metabolic rate, decreases in water intake, and increases in the contribution of metabolic water to the body water pool of Z. capensis, causing a consistent change in Δ’17O. Measurement of Δ’17O also provides an estimate of the δ18O composition of ingested pre-formed (drinking/food) water.
  • Estimated δ18O values of drinking/food water for captive Z. capensis were ~ -11‰, which is consistent with that of tap water in Santiago, Chile. In contrast, δ18O values of drinking/food water ingested by wild-caught Cinclodes were similar to that of seawater, which is consistent with their reliance on marine resources. Our results confirm the utility of this method for quantifying the relative contribution of metabolic versus pre-formed drinking/food water to the body water pool in birds.
isotope
isotope

Does the Amount of Stable Isotope Dose Influence Retinol Kinetic Responses and Predictions of Vitamin A Total Body Stores by the Retinol Isotope Dilution Method in Theoretical Children and Adults?

Background: To minimize both cost and perturbations to the vitamin A system, investigators limit the amount of stable isotope administered when estimating vitamin A total body stores (TBS) by retinol isotope dilution (RID).
Objectives: We hypothesized that reasonable increases in the mass of stable isotope administered to theoretical subjects would have only transient impacts on vitamin A kinetics and minimal effects on RID-predicted TBS.
Methods: We adapted previously-used theoretical subjects (3 children, 3 adults) with low, moderate, or high assigned TBS and applied compartmental analysis to solve a steady state model for tracer and tracee using assigned values for retinol kinetic parameters and plasma retinol.
To follow retinol trafficking when increasing amounts of stable isotope were administered [1.39-7 (children) and 2.8-14 µmol retinol (adults)], we added assumptions to an established compartmental model so that plasma retinol homeostasis was maintained.
Using model-simulated data, we plotted retinol kinetics versus time and applied the RID equation TBS = FaS/SAp [Fa, fraction of dose in stores; S, retinol specific activity (SA) in plasma/SA in stores; SAp, SA in plasma] to calculate vitamin A stores.
Results: The model predicted that increasing the stable isotope dose caused transient early increases in hepatocyte total retinol; increases in plasma tracer were accompanied by decreases in tracee to maintain plasma retinol homeostasis. Despite changes in kinetic responses, RID accurately predicted assigned TBS (98-105%) at all loads for all theoretical subjects from 1-28 d postdosing.
Conclusions: Results indicate that, compared with doses of 1.4-3.5 µmol used in recent RID field studies, doubling the stable isotope dose should not affect accuracy of TBS predictions, thus allowing for experiments of longer duration when including a super-subject design (Ford et al., J Nutr 2020;150:411-8) and/or studying retinol kinetics.
Keywords: model-based compartmental analysis; retinol isotope dilution method; retinol kinetics; theoretical humans; vitamin A status.
pAAV-RC9 vector
PVT12073 2 ug
EUR 524
pAAV-DJ vector
PVT12151 2 ug
EUR 438
pAAV-DJ Vector
VPK-420-DJ 10 µg
EUR 647
Description: The pAAV-DJ vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ packaging.
pAAV-RC1 Vector
VPK-421 10 µg
EUR 647
Description: The pAAV-RC1 vector contains the rep and cap genes required to generated recombinant AAV of serotype 1. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-1 packaging.
pAAV-RC3 Vector
VPK-423 10 µg
EUR 647
Description: The pAAV-RC3 vector contains the rep and cap genes required to generated recombinant AAV of serotype 3. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-3 packaging.
pAAV-RC4 Vector
VPK-424 10 µg
EUR 647
Description: The pAAV-RC4 vector contains the rep and cap genes required to generated recombinant AAV of serotype 4. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-4 packaging.
pAAV-RC5 Vector
VPK-425 10 µg
EUR 647
Description: The pAAV-RC5 vector contains the rep and cap genes required to generated recombinant AAV of serotype 5. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-5 packaging.
pAAV-RC6 Vector
VPK-426 10 µg
EUR 647
Description: The pAAV-RC6 vector contains the rep and cap genes required to generated recombinant AAV of serotype 6. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-6 packaging.
pAAV-RC6
PVT14647 2 ug
EUR 703
pAAV- RC
PVT2103 2 ug
EUR 241
pAAV-GFP Control Vector
AAV-400 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.
pAAV-Cre Control Vector
AAV-401 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.
pAAV-LacZ Control Vector
AAV-402 10 µg
EUR 566
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.
pAAV-MCS Expression Vector
VPK-410 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-DJ/8 Vector
VPK-420-DJ-8 10 µg
EUR 647
Description: The pAAV-DJ/8 vector contains the rep and cap genes required to generated recombinant AAV of serotype DJ/8. Co-transfect with other packaging plasmids and an expression vector into 293 cells for AAV-DJ/8 packaging.
pAAV- IRES- ZsGreen1
PVT11044 2 ug
EUR 301
pAAV- ZsGreen1- shRNA
PVT11045 2 ug
EUR 370
pAAV-fNPY-GFP
PVT14636 2 ug
EUR 599
pAAV-RSV-SpCas9
PVT17629 2 ug
EUR 300
pAAV-CAG-GFP
PVT17666 2 ug
EUR 341
pAAV- MCS Plasmid
PVT2102 2 ug
EUR 241
pAAV-MCS Promoterless Expression Vector
VPK-411 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-Puro Expression Vector
VPK-415 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-Neo Expression Vector
VPK-416 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-Hygro Expression Vector
VPK-417 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-GFP Expression Vector
VPK-418 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-IRES-Bsd Expression Vector
VPK-419 10 µg
EUR 647
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.
pAAV-EF1a-DIO-mCherry
PVT17841 2 ug
EUR 300
pAAV- IRES- hrGFP Plasmid
PVT2104 2 ug
EUR 266
Human Topoisomerase I
TG2005H-RC2 500 units
EUR 448
pAAV- CMV- mCherry- U6- sgRNA
PVT11046 2 ug
EUR 301
pAAV-hSyn-hChR2(H134R)-mCherry
PVT19026 2 ug
EUR 258
pAAV-hSyn-eNpHR 3.0-EYFP
PVT19063 2 ug
EUR 258
pAAV-EF1a-DIO-hM3D(Gq)-mCherry
PVT17847 2 ug
EUR 300
pAAV-MCS-Ppargc1a-m-FLAG-HA
PVT18341 2 ug
EUR 300
pVL1392 Vector
ABP-BVP-10001 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids
pVL1393 Vector
ABP-BVP-10002 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids
pORB Vector
ABP-BVP-10003 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids
pAcSec1 Vector
ABP-BVP-10004 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids
pAcIRES Vector
ABP-BVP-10005 5 ug Ask for price
    • Product line: Sapphire Baculovirus
    • Brand: Transfer Plasmids
pEE14.4 vector
PVT11901 2 ug
EUR 1036
pENTR223.1 vector
PVT11990 2 ug
EUR 352
pUB_smFLAG_KDM5B_MS2 vector
PVT11991 2 ug
EUR 352
ER2738 vector
PVT11993 2 ug
EUR 352
pFLPo vector
PVT12063 2 ug
EUR 352
pREDKI vector
PVT12064 2 ug
EUR 352
pREDCas9 vector
PVT12065 2 ug
EUR 352
PWUR790 vector
PVT12066 2 ug
EUR 352
xCas9 vector
PVT12068 2 ug
EUR 352
sgRNA vector
PVT12071 2 ug
EUR 352
PY094 vector
PVT12150 2 ug
EUR 352
PY094 vector
PVT12150-1 2 ug
EUR 352
pRGEB31 vector
PVT12152 2 ug
EUR 352
pSET152 vector
PVT3395 2 ug
EUR 376
pYLEX1 - Expression Vector
FYY203-5MG 5mg Ask for price
pYLSC1- Secretion Vector
FYY204-5MG 5mg Ask for price
pMRNAxp mRNAExpress Vector
MR000PA-1 10 ug
EUR 900
  • Category: Stem Cell Products
pMXs Retroviral Vector
RTV-010 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging
pMYs Retroviral Vector
RTV-020 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging
pMZs Retroviral Vector
RTV-030 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging
pENTR223-ATP5PO vector
PVT11685 2 ug
EUR 304
pENTR223- LC25A10 vector
PVT11686 2 ug
EUR 304
pENTR223-RSU1 vector
PVT11687 2 ug
EUR 304
pENTR223-CCDC24 vector
PVT11688 2 ug
EUR 304
pENTR223-BAT2L vector
PVT11689 2 ug
EUR 304