4 Host, 96T, apoptosis, array, Bacteria Pig Pigeon, Bafilomycin A1, Blocking, Ch 223191, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Goat, Green, Mip 1B, Pamabrom 100Mg, Pepstatin A, Phospho 4Ebp1, Plant, plasmid, Plate, Tubastatin A, Valproic Acid Sodium Salt

Mass Measurements of Neutron-Deficient Yb Isotopes and Nuclear Structure at the Extreme Proton-Rich Side of the N=82 Shell

High-accuracy mass measurements of neutron-deficient Yb isotopes have been performed at TRIUMF using TITAN’s multiple-reflection time-of-flight mass spectrometer (MR-TOF-MS). For the first time, an MR-TOF-MS was used on line simultaneously as an isobar separator and as a mass spectrometer, extending the measurements to two isotopes further away from stability than otherwise possible.
The ground state masses of ^{150,153}Yb and the excitation energy of ^{151}Yb^{m} were measured for the first time. As a result, the persistence of the N=82 shell with almost unmodified shell gap energies is established up to the proton drip line. Furthermore, the puzzling systematics of the h_{11/2}-excited isomeric states of the N=81 isotones are unraveled using state-of-the-art mean field calculations.

The CERN-MEDICIS Isotope Separator Beamline

CERN-MEDICIS is an off-line isotope separator facility for the extraction of radioisotopes from irradiated targets of interest to medical applications. The beamline, between the ion source and the collection chamber, consists of ion extraction and focusing elements, and a dipole magnet mass spectrometer recovered from the LISOL facility in Louvain-la-Neuve.
The latter has been modified for compatibility with MEDICIS, including the installation of a window for injecting laser light into the ion source for resonance photo-ionization. Ion beam optics and magnetic field modeling using SIMION and OPERA respectively were performed for the design and characterization of the beamline. The individual components and their optimal configuration in terms of ion beam extraction, mass separation, and ion transport efficiency is described, along with details of the commissioning and initial performance assessment with stable ion beams.

Triple Oxygen Isotope Measurements (Δ’ 17 O) of Body Water Reflect Water Intake, Metabolism, and δ 18 O of Ingested Water in Passerines

Understanding physiological traits and ecological conditions that influence a species reliance on metabolic water is critical to creating accurate physiological models that can assess their ability to adapt to environmental perturbations (e.g., drought) that impact water availability. However, relatively few studies have examined variation in the sources of water animals use to maintain water balance, and even fewer have focused on the role of metabolic water. A key reason is methodological limitations.
Here, we applied a new method that measures the triple oxygen isotopic composition of a single blood sample to estimate the contribution of metabolic water to the body water pool of three passerine species. This approach relies on Δ’17O, defined as the residual from the tight linear correlation that naturally exists between δ17O and δ18O values. Importantly, Δ’17O is relatively insensitive to key fractionation processes, such as Rayleigh distillation in the water cycle that have hindered previous isotope-based assessments of animal water balance.
  • We evaluated the effects of changes in metabolic rate and water intake on Δ’17O values of captive rufous-collared sparrows (Zonotrichia capensis) and two invertivorous passerine species in the genus Cinclodes from the field.
  • As predicted, colder acclimation temperatures induced increases in metabolic rate, decreases in water intake, and increases in the contribution of metabolic water to the body water pool of Z. capensis, causing a consistent change in Δ’17O. Measurement of Δ’17O also provides an estimate of the δ18O composition of ingested pre-formed (drinking/food) water.
  • Estimated δ18O values of drinking/food water for captive Z. capensis were ~ -11‰, which is consistent with that of tap water in Santiago, Chile. In contrast, δ18O values of drinking/food water ingested by wild-caught Cinclodes were similar to that of seawater, which is consistent with their reliance on marine resources. Our results confirm the utility of this method for quantifying the relative contribution of metabolic versus pre-formed drinking/food water to the body water pool in birds.
isotope
isotope

Does the Amount of Stable Isotope Dose Influence Retinol Kinetic Responses and Predictions of Vitamin A Total Body Stores by the Retinol Isotope Dilution Method in Theoretical Children and Adults?

Background: To minimize both cost and perturbations to the vitamin A system, investigators limit the amount of stable isotope administered when estimating vitamin A total body stores (TBS) by retinol isotope dilution (RID).
Objectives: We hypothesized that reasonable increases in the mass of stable isotope administered to theoretical subjects would have only transient impacts on vitamin A kinetics and minimal effects on RID-predicted TBS.
Methods: We adapted previously-used theoretical subjects (3 children, 3 adults) with low, moderate, or high assigned TBS and applied compartmental analysis to solve a steady state model for tracer and tracee using assigned values for retinol kinetic parameters and plasma retinol.
To follow retinol trafficking when increasing amounts of stable isotope were administered [1.39-7 (children) and 2.8-14 µmol retinol (adults)], we added assumptions to an established compartmental model so that plasma retinol homeostasis was maintained.
Using model-simulated data, we plotted retinol kinetics versus time and applied the RID equation TBS = FaS/SAp [Fa, fraction of dose in stores; S, retinol specific activity (SA) in plasma/SA in stores; SAp, SA in plasma] to calculate vitamin A stores.
Results: The model predicted that increasing the stable isotope dose caused transient early increases in hepatocyte total retinol; increases in plasma tracer were accompanied by decreases in tracee to maintain plasma retinol homeostasis. Despite changes in kinetic responses, RID accurately predicted assigned TBS (98-105%) at all loads for all theoretical subjects from 1-28 d postdosing.
Conclusions: Results indicate that, compared with doses of 1.4-3.5 µmol used in recent RID field studies, doubling the stable isotope dose should not affect accuracy of TBS predictions, thus allowing for experiments of longer duration when including a super-subject design (Ford et al., J Nutr 2020;150:411-8) and/or studying retinol kinetics.
Keywords: model-based compartmental analysis; retinol isotope dilution method; retinol kinetics; theoretical humans; vitamin A status.

pAAV-RC2 Vector

MBS169538-5x001mg 5x0.01mg
EUR 2955

pAAV-RC2

PVT35966-1 2ug
EUR 280

pAAV-RC1 Vector

VPK-421 10 µg
EUR 530

pAAV-RC3 Vector

VPK-423 10 µg
EUR 530

pAAV-RC4 Vector

VPK-424 10 µg
EUR 530

pAAV-RC5 Vector

VPK-425 10 µg
EUR 530

pAAV-RC6 Vector

VPK-426 10 µg
EUR 530

pAAV-RC1 Vector

MBS169537-001mg 0.01mg
EUR 695

pAAV-RC1 Vector

MBS169537-5x001mg 5x0.01mg
EUR 2955

pAAV-RC3 Vector

MBS169539-001mg 0.01mg
EUR 695

pAAV-RC3 Vector

MBS169539-5x001mg 5x0.01mg
EUR 2955

pAAV-RC4 Vector

MBS169540-001mg 0.01mg
EUR 695

pAAV-RC4 Vector

MBS169540-5x001mg 5x0.01mg
EUR 2955

pAAV-RC5 Vector

MBS169541-001mg 0.01mg
EUR 695

pAAV-RC5 Vector

MBS169541-5x001mg 5x0.01mg
EUR 2955

pAAV-RC6 Vector

MBS169542-001mg 0.01mg
EUR 695

pAAV-RC6 Vector

MBS169542-5x001mg 5x0.01mg
EUR 2955

pAAV-RC9 vector

PVT12073 2ug
EUR 408

pAAV-DJ Vector

VPK-420-DJ 10 µg
EUR 530

pAAV-DJ Vector

MBS169535-001mg 0.01mg
EUR 695

pAAV-DJ Vector

MBS169535-5x001mg 5x0.01mg
EUR 2955

pAAV-DJ vector

PVT12151 2ug
EUR 325

pAAV-DJ/8 Vector

VPK-420-DJ-8 10 µg
EUR 530

pAAV-DJ/8 Vector

MBS169536-001mg 0.01mg
EUR 695

pAAV-DJ/8 Vector

MBS169536-5x001mg 5x0.01mg
EUR 2955

pAAV-GFP Control Vector

AAV-400 10 µg
EUR 455

pAAV-Cre Control Vector

AAV-401 10 µg
EUR 679.2
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-GFP Control Vector

MBS168881-001mg 0.01mg
EUR 610

pAAV-GFP Control Vector

MBS168881-5x001mg 5x0.01mg
EUR 2575

pAAV-LacZ Control Vector

AAV-402 10 µg
EUR 679.2
Description: Use this control vector to co-transfect along with AAV packaging vectors to produce a recombinant AAV control.

pAAV-MCS Expression Vector

VPK-410 10 µg
EUR 530

pAAV-MCS Expression Vector

MBS169528-001mg 0.01mg
EUR 695

pAAV-MCS Expression Vector

MBS169528-5x001mg 5x0.01mg
EUR 2955

pAAV-IRES-Neo Expression Vector

VPK-416 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

VPK-418 10 µg
EUR 530

pAAV-IRES-Bsd Expression Vector

VPK-419 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-GFP Expression Vector

MBS169533-001mg 0.01mg
EUR 695

pAAV-IRES-GFP Expression Vector

MBS169533-5x001mg 5x0.01mg
EUR 2955

pAAV-IRES-Puro Expression Vector

VPK-415 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-IRES-Hygro Expression Vector

VPK-417 10 µg
EUR 776.4
Description: Clone your gene of interest into this AAV Expression Vector, then co-transfect along with AAV packaging vectors into a packaging host cell line such as 293AAV.

pAAV-MCS Promoterless Expression Vector

VPK-411 10 µg
EUR 530

pAAV-MCS Promoterless Expression Vector

MBS169529-001mg 0.01mg
EUR 695

pAAV-MCS Promoterless Expression Vector

MBS169529-5x001mg 5x0.01mg
EUR 2955

Green Kit. Baculovirus GFP vector.

K20 1 Kit
EUR 695
Description: Protein expression

ProGreen. Baculovirus GFP marker vector.

A1 25 ul
EUR 420
Description: Protein expression

pVL1393. General baculovirus plasmid vector.

B1 50 ul
EUR 340
Description: Protein expression

ProEasy. Vector for easy construction of recombinant baculoviruses.

A10S 25 ul
EUR 695
Description: Protein expression

pAcAB3. Baculovirus plasmid vector for expression of up to 3 proteins.

B2 50 ul
EUR 420
Description: Protein expression

pAB-bee. Baculovirus plasmid vector for secreted and transmembrane proteins.

B3 50 ul
EUR 495
Description: Protein expression

ProFold-PDI. Baculovirus chaperone vector for expression of cysteine-rich proteins.

A7 25 ul
EUR 830
Description: Protein expression

ProFold-C1. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins.

A2 25 ul
EUR 830
Description: Protein expression

ProFold-C2. Baculovirus chaperone vector for expression of cytoplasmic and nuclear proteins.

A3 25 ul
EUR 830
Description: Protein expression

ProFold-ER1. Baculovirus chaperone vector for expression of secreted and membrane proteins.

A4 25 ul
EUR 830
Description: Protein expression

pAAV-RC6

PVT14647 2ug
EUR 580

C1 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins.

K21 1 Kit
EUR 995
Description: Protein expression

C2 Kit. Baculovirus chaperone vectors for cytoplasmic and nuclear proteins.

K22 1 Kit
EUR 995
Description: Protein expression

ER1 Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins.

K23 1 Kit
EUR 995
Description: Protein expression

ER1-bee Kit. Baculovirus chaperone vectors for expression of secreted and membrane proteins.

K24 1 Kit
EUR 995
Description: Protein expression

pAAV-RC

PVTY00744 2ug
EUR 280

pAAV- RC

PVT2103 2ug
EUR 162

pAAV- MCS

PVT2102 2ug
EUR 162

pAAV-MCS

PVTY00902 2ug
EUR 280

pAAV-GFP

PVT23853 2ug
EUR 280

pAAV-LacZ

PVTY00602 2ug
EUR 280

pAAV-hrGFP

PVTY00600 2ug
EUR 280
Andrew Green