4 Host, 96T, apoptosis, array, Assay, Bacteria Pig Pigeon, Bafilomycin A1, Blocking, Ch 223191, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Green, Mip 1B, Pepstatin A, Phospho 4Ebp1, Plant, Plate

Determination of Picomolar Titanium in Seawater by Isotope Dilution Multicollector Inductively Coupled Plasma Mass Spectrometry after Mg(OH) 2 Coprecipitation

A brand new isotope dilution inductively coupled plasma mass spectrometry (ICPMS) technique is developed to find out picomolar concentrations of titanium (Ti) in seawater. The strategy applies Mg(OH)2 coprecipitation to pay attention Ti from seawater, and makes use of a brand new 49Ti-47Ti isotope dilution to remove the necessity for separating Ti from seawater Ca, leading to an isobaric interference-free evaluation by high-resolution multicollector ICPMS. The strategy makes use of a 1.eight mL seawater pattern with a detection restrict of 1.6 pmol L-1 that’s decided primarily by Ti contamination throughout pattern preparation reasonably than by ICPMS sensitivity, instrumental Ti background, or isobaric interferences. An oceanographically constant vertical profile of dissolved Ti within the Sargasso Sea close to Bermuda is measured with this technique.

Potassium isotope composition of Mars reveals a mechanism of planetary unstable retention

The abundances of water and extremely to reasonably unstable components in planets are thought of vital to mantle convection, floor evolution processes, and habitability. From the primary flyby area probes to the more moderen “Perseverance” and “Tianwen-1” missions, “comply with the water,” and, extra broadly, “volatiles,” has been one of many key themes of martian exploration. Ratios of volatiles relative to refractory components (e.g., Okay/Th, Rb/Sr) are in step with a better unstable content material for Mars than for Earth, regardless of the contrasting present-day floor situations of these our bodies.
This examine presents Okay isotope knowledge from a spectrum of martian lithologies as an isotopic tracer for evaluating the inventories of extremely and reasonably unstable components and compounds of planetary our bodies. Right here, we present that meteorites from Mars have systematically heavier Okay isotopic compositions than the majority silicate Earth, implying a larger lack of Okay from Mars than from Earth.
The common “bulk silicate” δ41Okay values of Earth, Moon, Mars, and the asteroid 4-Vesta correlate with floor gravity, the Mn/Na “volatility” ratio, and most notably, bulk planet H2O abundance. These relationships point out that planetary unstable abundances consequence from variable unstable loss throughout accretionary development during which bigger mass our bodies preferentially retain unstable components over decrease mass objects. There may be probably a threshold on the scale necessities of rocky (exo)planets to retain sufficient H2O to allow habitability and plate tectonics, with mass exceeding that of Mars.

The genetic structure of leaf secure carbon isotope composition in Zea mays and the impact of transpiration effectivity on leaf elemental accumulation

With elevated demand on freshwater assets for agriculture, it’s crucial that extra water-use environment friendly crops are developed. Leaf secure carbon isotope composition, δ13C, is a proxy for transpiration effectivity and a doable instrument for breeders, however the underlying mechanisms effecting δ13C in C4 vegetation aren’t identified.
It has been urged that variations in particular leaf space (SLA), which doubtlessly displays variation in inside CO2 diffusion, can impression leaf δ13C. Moreover, though it’s identified that water motion is essential for elemental uptake, it’s not clear how manipulation of transpiration for elevated water-use effectivity could impression nutrient accumulation. Right here, we characterize the genetic structure of leaf δ13C and take a look at its relationship to SLA and the ionome in 5 populations of maize.
5 important QTL for leaf δ13C had been recognized, together with novel QTL in addition to some that had been recognized beforehand in maize kernels. One of many QTL areas incorporates an Erecta-like gene, the ortholog of which has been proven to manage transpiration effectivity and leaf δ13C in Arabidopsis. QTL for δ13C had been positioned in the identical normal chromosome area, however barely shifted, when evaluating knowledge from two totally different years.
Our knowledge doesn’t assist a relationship between δ13C and SLA, and of the 19 components analyzed, solely a weak correlation between molybdenum and δ13C was detected. Collectively these knowledge add to the genetic understanding of leaf δ13C in maize and counsel that enhancements to plant water use could also be doable with out considerably influencing elemental homeostasis.

Measuring Cyanobacterial Metabolism in Biofilms with NanoSIMS Isotope Imaging and Scanning Electron Microscopy (SEM)

To advance the understanding of microbial interactions, it’s changing into more and more essential to resolve the person metabolic contributions of microorganisms in complicated communities. Organisms from biofilms might be particularly troublesome to separate, picture and analyze, and strategies to deal with these limitations are wanted.
Excessive-resolution imaging secondary ion mass spectrometry (NanoSIMS) generates single cell isotopic composition measurements, and can be utilized to quantify incorporation and change of an isotopically labeled substrate amongst particular person organisms.
Right here, incorporation of cyanobacterial extracellular natural matter (EOM) by members of a cyanobacterial combined species biofilm is used as a mannequin as an example this technique. Incorporation of secure isotope labeled (15N and 13C) EOM by two teams, cyanobacteria and related heterotrophic microbes, are quantified. Strategies for producing, making ready, and analyzing samples for quantifying uptake of secure isotope-labeled EOM within the biofilm are described.

 

Pseudo isotope dilution (PID) as an strategy for correcting barium-related spectral interferences on the measurement of europium by inductively coupled plasma mass spectrometry (ICP-MS)

Pseudo isotope dilution (PID) was urged as a easy and sensible strategy for the correction of barium-related spectral interferences on the measurement of europium by inductively coupled plasma mass spectrometry (ICP-MS). The interferents, i.e. oxides and hydroxides of barium, had been thought to be pseudo europium isotopes. An equation for calculating the focus of europium was deduced and the web contribution of europium isotopes might be obtained by measuring the sign depth ratios of 151I/153I in a pattern, a europium normal, and a barium normal, respectively.
The validity of the strategy was confirmed by the dedication of europium in normal options with totally different concentrations of barium. Dedication of europium in river water and seawater licensed reference supplies was carried out, whose outcomes had been in coincidence with reference values.
isotope
isotope

Metabolic Heavy Isotope Labeling to Examine Glycerophospholipid Homeostasis of Cultured Cells

Glycerophospholipids encompass a glycerophosphate spine to that are esterified two acyl chains and a polar head group. The pinnacle group (e.g., choline, ethanolamine, serine or inositol) defines the glycerophospholipid class, whereas the acyl chains along with the pinnacle group outline the glycerophospholipid molecular species.
Steady heavy isotope (e.g., deuterium)-labeled head group precursors added to the tradition medium incorporate effectively into glycerophospholipids of mammalian cells, which permits one to find out the charges of synthesis, acyl chain reworking or turnover of the person glycerophospholipids utilizing mass spectrometry.
This protocol describes how you can examine the metabolism of the main mammalian glycerophospholipids i.e., phosphatidylcholines, phosphatidylethanolamines, phosphatidylserines and phosphatidylinositols with this strategy.

pBABE-Neo-hTERT Retroviral Vector

RTV-005 100 µL
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging

pBABE-Hygro-hTERT Retroviral Vector

RTV-007 100 µL
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging

pBABE-Hygro Retroviral Vector

RTV-001-HYGRO 10 ?g
EUR 566
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pBABE-GFP Retroviral Vector

RTV-002 10 ?g
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging

pBABE-Neo Retroviral Vector

RTV-003 100 µL
EUR 566
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pBABE-Zeo Retroviral Vector

RTV-004 100 µL
EUR 566
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

hTERT (CMV, Puro) lentivirus

LVP1130-Puro 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under suCMV promoter, containing Puromycin marker.

hTERT (EF1a, Puro) lentivirus

LVP1131-Puro 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under EF1a promoter, containing Puromycin marker.

pBABE-hygro-hTERT

PVT17568 2 ug
EUR 341

pBABE- neo- hTERT

PVT10354 2 ug
EUR 301

hTERT (CMV, Puro) lentivirus in PBS

LVP1130-Puro-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing Puromycin marker.

hTERT (EF1a, Puro) lentivirus in PBS

LVP1131-Puro-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under EF1a promoter, containing Puromycin marker.

pBABE- Puro

PVT11054 2 ug
EUR 301

pMXs-Puro Retroviral Vector

RTV-012 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMYs-Puro Retroviral Vector

RTV-024 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMCs-Puro Retroviral Vector

RTV-041 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-IRES-Puro Retroviral Vector

RTV-014 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMYs-IRES-Puro Retroviral Vector

RTV-022 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-GFP/Puro Retroviral Vector

RTV-053 10 µg
EUR 717
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-EF1-Puro Retroviral Vector

RTV-060 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-Cas9-Puro Retroviral Vector

RTV-5106 100 µL
EUR 717

pBABE-Puro-Sam68 Plasmid

PVTB00471-4a 2 ug
EUR 356

pBABE-Puro-ARHGEF4 Plasmid

PVTB00766-4a 2 ug
EUR 356

pBABE-Puro-CNOT1 Plasmid

PVTB00869-2a 2 ug
EUR 356

pBABE-Puro-PPP2R5A Plasmid

PVTB01072-4a 2 ug
EUR 356

pBABE puro-EGFP Plasmid

PVTB80003-4a 2 ug
EUR 356

pBABE-Puro-HA-Brca1

PVT17834 2 ug
EUR 424

pBABE- Puro SV40 LT

PVT10529 2 ug
EUR 301

pBABE-Puro-hTID-1 Plasmid

PVTB00190-4a 2 ug
EUR 356

pBABE-Puro-KIFAP3-HA Plasmid

PVTB01071-4b 2 ug
EUR 356

pBABE-Puro-AP-2 Plasmid

PVTB01073-4a 2 ug
EUR 356

pBABE-puro mCherry-EGFP-LC3B

PVT14018 2 ug
EUR 911

HA- HIF1alpha- wt- pBabe- puro

PVT10257 2 ug
EUR 301

pMXs Retroviral Vector

RTV-010 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMYs Retroviral Vector

RTV-020 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMZs Retroviral Vector

RTV-030 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-miRNA-GFP/Puro Retroviral Expression Vector

RTV-017 10 ?g
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

hTERT (CMV, GFP-Puro) lentivirus

LVP1130-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under suCMV promoter, containing GFP-Puromycin fusion dual marker.

hTERT (CMV, RFP-Puro) lentivirus

LVP1130-RP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under suCMV promoter, containing RFP-Puromycin marker.

hTERT (EF1a, GFP-Puro) lentivirus

LVP1131-GP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under EF1a promoter, containing GFP-Puromycin fusion dual marker.

hTERT (EF1a, RFP-Puro) lentivirus

LVP1131-RP 1x107 IFU/ml x 200ul
EUR 349
Description: Premade lentivirus expressing human TERT gene under EF1a promoter, containing RFP-Puromycin marker.

pBABE-Puro-IQGAP1(1503-1657aa) Plasmid

PVTB01069-4a 2 ug
EUR 356

pBABE-Puro-Striatin(366-731aa) Plasmid

PVTB01070-4a 2 ug
EUR 356

pBABE-Puro-KIFAP3(606-772aa) Plasmid

PVTB01071-4a 2 ug
EUR 356

pBABE-Puro-AMER1(716-834aa) Plasmid

PVTB01079-4a 2 ug
EUR 356

HEK293-TetR (Puro)

SC005-Puro 2 x 106 cell/ml x 1ml
EUR 1210
Description: tetracycline repressor in HEK293 stable cell line with Puromycin fusion marker

pBABE- hLin28B

PVT10399 2 ug
EUR 301

pBABE GFP

PVT11055 2 ug
EUR 266

pWZL-Neo Retroviral Vector

RTV-001-NEO 10 ?g
EUR 566
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-Neo Retroviral Vector

RTV-011 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMX-GFP Retroviral Vector

RTV-050 10 µg
EUR 717
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMCs-GFP Retroviral Vector

RTV-051 10 µg
EUR 717
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMYs-GFP Retroviral Vector

RTV-052 10 µg
EUR 717
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-EF1? Retroviral Vector

RTV-063 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-CAG Retroviral Vector

RTV-064 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-CMV Retroviral Vector

RTV-065 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-SR? Retroviral Vector

RTV-066 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

rtTA (Puro) , Lentiviral particles

LVP400-Puro 1x107 IFU/ml x 200ul
EUR 404
Description: Pre-made lentiviral particles expressing reverse tetracycline Transcriptional Activator (rtTA) under enhanced EF1a promoter with Puromycin antibioic marker, provided in DMEM medium with 10% FBS.

hTERT Antibody

20-abx120550
  • EUR 328.00
  • EUR 105.00
  • EUR 133.00
  • 100 ug
  • 10 ug
  • 25 ug

hTERT Antibody

abx122247-100ug 100 ug
EUR 391

hTERT Peptide

20-abx069990
  • EUR 182.00
  • EUR 84.00
  • 125 ul
  • 25 ul

Adeno-hTERT

G205 1.0 ml
EUR 1014

Hela-TetR (Puro) stable cells

SC035-Puro 2 x 106 cell/ml x 1ml
EUR 1210
Description: tetracycline repressor expression Hela stable cell line with Puromycin marker

CMV-TetR (Puro) Lentiviral particles 

LVP017-Puro 1x107 IFU/ml x 200ul
EUR 404
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under CMV promoter, with Puromycin antibiotic marker.

β-Lactamase (Puro) lentiviral particles

LVP335-Puro 1x107 IFU/ml x 200ul
EUR 349
Description: pre-made lentiviral particles expressing β-Lactamase gene. A puromycin marker was expressed under RSV promoter. Particles is provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

EF1a-TetR (Puro) Lentiviral particles 

LVP459-Puro 1x107 IFU/ml x 200ul
EUR 404
Description: Pre-made lentiviral particles expressing tetracycline regulator protein (TetR) under enhanced EF1a promoter with Puromycin antibioic marker.

LoxP GFP/RFP ColorSwitch (Puro) lentivirus

LVP460-Puro 1x107 IFU/ml x 200ul
EUR 710
Description: Pre-made lentiviral particles expressing "LoxP-GFP-stop-LoxP-RFP-Stop" cassette under suCMV promoter. It also contains a puromycin antibiotic selection marker under Rsv promoter. Virus is provided in DMEM medium with 10% FBS and 60ug/ml of polybrene.

hTERT (CMV, GFP-Puro) lentivirus in PBS

LVP1130-GP-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing GFP-Puromycin fusion dual marker.

hTERT (CMV, RFP-Puro) lentivirus in PBS

LVP1130-RP-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under suCMV promoter, containing RFP-Puromycin marker.

hTERT (EF1a, GFP-Puro) lentivirus in PBS

LVP1131-GP-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under EF1a promoter, containing GFP-Puromycin fusion dual marker.

hTERT (EF1a, RFP-Puro) lentivirus in PBS

LVP1131-RP-PBS 1x108 IFU/ml x 200ul
EUR 710
Description: Concentrated lentivirus in PBS, expressing human TERT gene under EF1a promoter, containing RFP-Puromycin marker.

Lenti-CMV-hTERT-RFP-2A-Puro Virus

LV625 10 ml
EUR 1059

Lenti-CMV-hTERT-GFP-2A-Puro Virus

LV623 10 ml
EUR 1059

pMXs-IRES-GFP Retroviral Vector

RTV-013 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-IRES-Neo Retroviral Vector

RTV-015 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-IRES-Bsd Retroviral Vector

RTV-016 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMYs-IRES-GFP Retroviral Vector

RTV-021 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMYs-IRES-Neo Retroviral Vector

RTV-023 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMCs-IRES-GFP Retroviral Vector

RTV-040 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-EF1-GFP Retroviral Vector

RTV-061 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pMXs-EF1-Bsd Retroviral Vector

RTV-062 10 µg
EUR 624
Description: Use this construct to clone your gene for downstream recombinant retroviral packaging

pBABEpuro-Ras V12 Retroviral Vector

RTV-101 100 µL
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging

pBABEpuro-myr-Rac1 Retroviral Vector

RTV-201 100 µL
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging

pBABEhygro-Rac1 V12 Retroviral Vector

RTV-202 100 µL
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging

pBABEhygro-Cdc42 L61 Retroviral Vector

RTV-203 100 µL
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging

pBABEhygro-RhoA L63 Retroviral Vector

RTV-204 100 µL
EUR 717
Description: Use this gene-specific construct for downstream recombinant retroviral packaging