4 Host, 96T, apoptosis, array, Assay, Bacteria Pig Pigeon, Bafilomycin A1, Blocking, Ch 223191, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Goat, Green, Guinea, Mip 1B, Pamabrom 100Mg, Pepstatin A, Phospho 4Ebp1, Tubastatin A
Copper Isotopes and Copper to Zinc Ratio as Possible Biomarkers for Thyroid Cancer
Thyroid cancer is the most common endocrine cancer. There is no systematic screening for such cancer, and the current challenge is to find potential biomarkers to facilitate an early diagnosis. Copper (Cu) and zinc (Zn) are essential micronutrients involved in the proper functioning of the thyroid gland, and changes in their concentrations have been observed in the development of cancer.
Previous studies have highlighted the potential 65Cu/63Cu ratio (δ65Cu) to be a cancer biomarker. This study tests its sensitivity on plasma samples (n = 46) of Algerian patients with papillary thyroid carcinoma and a set of corresponding biopsies (n = 11). The δ65Cu ratio in blood and tumor samples was determined using multi collector inductively coupled plasma-mass spectrometry (MC-ICP-MS), and their corresponding Cu and Zn plasma total concentrations using total reflection X-ray fluorescence (TXRF).
Plasma concentrations of Cu were significantly higher (1346.1 ± 328.3 vs. 1060.5 ± 216.1 μg/L, p < 0.0001), and Zn significantly lower (942.1 ± 205.2 vs. 1027.9 ± 151.4 μg/L, p < 0.05) in thyroid cancer patients as compared to healthy controls (n = 50). Accordingly, the Cu/Zn ratio was significantly different between patients and controls (1.5 ± 0.4 vs. 1.0 ± 0.3, p < 0.0001). Furthermore, the δ65Cu plasma levels of patients were significantly lower than healthy controls (p < 0.0001), whereas thyroid tumor tissues presented high δ65Cu values. These results support the hypothesis that Cu isotopes and plasma trace elements may serve as suitable biomarkers of thyroid cancer diagnosis.
Visualization of DNA replication in single chromosome by stable isotope labeling
Among the inheritance of cellular components during cell division, deoxyribonucleic acid (DNA) and its condensate (chromosome) are conventionally visualized using chemical tag-labeled nucleotide analogs. However, associated mutagenesis with nucleotide analogs in the visualization of chromosomes is cause for concern. This study investigated the efficiency of using stable isotope labels in visualizing the replicating cultured human cell-chromosomes, in the absence of analog labels, at a high spatial resolution of 100 nm.
The distinct carbon isotope ratio between sister chromatids reflected the semi-conservative replication of individual DNA strands through cell cycles and suggested the renewal of histone molecules in daughter chromosomes. Thus, this study provides a new, powerful approach to trace and visualize cellular components with stable-isotope labeling.Key Words: stable isotope, chromosome replication, semi-conservative replication, imaging, mass spectrometry.
A high temperature water vapor equilibration method to determine non-exchangeable hydrogen isotope ratios of sugar, starch, and cellulose
The analysis of the non-exchangeable hydrogen isotope ratio (δ2 Hne ) in carbohydrates is mostly limited to the structural component cellulose, while simple high-throughput methods for δ2 Hne values of non-structural carbohydrates (NSC) such as sugar and starch do not yet exist. Here we tested if the hot vapor equilibration method originally developed for cellulose is applicable for NSC, verified by comparison with the traditional nitration method.
We set up a detailed analytical protocol and applied the method to plant extracts of leaves from species with different photosynthetic pathways (i.e., C3 , C4 , CAM). δ2 Hne of commercial sugars and starch from different classes and sources, ranging from -157.8 to +6.4‰, were reproducibly analysed with a precision between 0.2 and 7.7‰. Mean δ2 Hne values of sugar are lowest in C3 (-92.0‰), intermediate in C4(-32.5‰), and highest in CAM plants (6.0‰), with NSC being 2 H-depleted compared to cellulose and sugar being generally more 2 H-enriched than starch.
Our results suggest that our method can be used in future studies to disentangle 2 H-fractionation processes, for improving mechanistic δ2 Hne models for leaf and tree-ring cellulose, and for further development of δ2 Hne in plant carbohydrates as a potential proxy for climate, hydrology, plant metabolism and physiology. This article is protected by copyright. All rights reserved.
Rapid analysis of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) isotopologues in stable isotope-resolved metabolomics (SIRM) using direct infusion nanoelectrospray ultra-high-resolution Fourier transform mass spectrometry (DI-nESI-UHR-FTMS)
S-Adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are important metabolites in the one-carbon cycle that modulates cellular methylation required for proliferation and epigenetic regulation. Their concentrations, synthesis, and turnover are difficult to determine conveniently and reliably. We have developed such a method by coupling a simple and rapid purification scheme that efficiently captures both compounds, with high sensitivity, sample throughput direct infusion nanoelectrospray ultra-high-resolution Fourier transform mass spectrometry (DI-nESI-UHR-FTMS). This method is compatible with Stable Isotope-Resolved Metabolomic (SIRM) analysis of numerous other metabolites.
The limits of detection for both SAM and SAH were <1 nM, and the linearity range was up to 1000 nM. The method was first illustrated for SAM/SAH analysis of mouse livers, and lung adenocarcinoma A549 cells. We then applied the method to track 13C1-CH3-Met incorporation into SAM and 13C6-glucose transformation into SAM and SAH via de novo synthesis. We further used the method to show the distinct effects on A549 and H1299 cells with treatment of anti-cancer methylseleninic acid (MSA), selenite, and selenomethionine, notably SAM depletion and increased SAM to SAH ratio by MSA, which implicates altered epigenetic regulation.
Novel nickel isolation procedure for a wide range of sample matrices without using dimethylglyoxime for isotope measurements using MC-ICP-MS
Nickel isotope ratios have traditionally been used as an important tracer in cosmochemistry, and recently, it has gained attention in geochemistry, biochemistry, and environmental sciences with the development of MC-ICP-MS. Purification of Ni before isotope measurement is mandatory for obtaining precise data, which has been commonly achieved with ion-exchange chromatography, employing dimethylglyoxime (DMG) as a chelating agent for Ni.
- However, it has been pointed out that the use of DMG can adversely affect the isotope measurement due to insufficient Ni recovery and mass bias during measurement caused by the remaining DMG. Ni isolation procedures without the usage of DMG were innovated, but they have disadvantages such as the necessity of complex separation methods, high Ni blank, and matrix-dependent Ni recovery.
- Here, we present a simple Ni isolation procedure without using DMG but with the aid of oxalic acid along with common inorganic acids, achieving near-complete recovery of Ni with low blanks [0.7 ± 0.3 ng (2SD, n = 4)] only using three ion exchange column steps.
- To validate our method and strengthen the Ni isotope database of reference materials, 60Ni/58Ni of 20 geological reference materials, covering wide matrix compositions, were measured by MC-ICP-MS using the double-spike method.
- The results have shown that high recovery of Ni, independent of the sample matrix elements was achieved (98 ± 4%) and the 60Ni/58Ni was measured with a 2SD of 0.006-0.084‰ from samples containing 100-200 ng Ni.
) Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423157-01mg | MyBiosource | 0.1mg | EUR 610 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423157-025mg | MyBiosource | 0.25mg | EUR 1035 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423157-05mg | MyBiosource | 0.5mg | EUR 1320 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423158-002mg | MyBiosource | 0.02mg | EUR 210 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423158-01mg | MyBiosource | 0.1mg | EUR 365 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423158-025mg | MyBiosource | 0.25mg | EUR 1035 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423158-05mg | MyBiosource | 0.5mg | EUR 1320 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423158-1mg | MyBiosource | 1mg | EUR 1775 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423159-002mg | MyBiosource | 0.02mg | EUR 210 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423159-01mg | MyBiosource | 0.1mg | EUR 365 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423159-025mg | MyBiosource | 0.25mg | EUR 1035 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423159-05mg | MyBiosource | 0.5mg | EUR 1460 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423159-1mg | MyBiosource | 1mg | EUR 1775 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423160-002mg | MyBiosource | 0.02mg | EUR 210 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423160-01mg | MyBiosource | 0.1mg | EUR 365 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423160-025mg | MyBiosource | 0.25mg | EUR 1035 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423160-05mg | MyBiosource | 0.5mg | EUR 1320 |
Recombinant Human Parathyroid hormone protein (PTH) |
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| MBS9423160-1mg | MyBiosource | 1mg | EUR 1775 |
 (Active)) Recombinant Human Parathyroid hormone protein(PTH) (Active) |
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| AP73570 | SAB | 1mg | EUR 1968 |
Recombinant Human Parathyroid hormone protein(PTH) (Active) |
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| AP73571 | SAB | 1mg | EUR 1968 |
Recombinant Human Parathyroid hormone protein(PTH) (Active) |
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| AP73572 | SAB | 1mg | EUR 1968 |
 (Active)) Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| CSB-AP000051HU | Cusabio | 8497 mg | Ask for price |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969120-01mg | MyBiosource | 0.1mg | EUR 530 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969120-05mg | MyBiosource | 0.5mg | EUR 1080 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969120-5x05mg | MyBiosource | 5x0.5mg | EUR 4595 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969121-01mg | MyBiosource | 0.1mg | EUR 530 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969121-05mg | MyBiosource | 0.5mg | EUR 1080 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969121-5x05mg | MyBiosource | 5x0.5mg | EUR 4595 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969123-01mg | MyBiosource | 0.1mg | EUR 530 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969123-05mg | MyBiosource | 0.5mg | EUR 1080 |
Recombinant Human Parathyroid hormone protein (PTH) (Active) |
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| MBS969123-5x05mg | MyBiosource | 5x0.5mg | EUR 4595 |
) Recombinant Human Parathyroid Hormone/PTH Protein (1-84) |
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| PKSH032854-10ug | Elabscience Biotech | 10ug | EUR 129 |
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Description: Human |
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Recombinant Human Parathyroid Hormone/PTH Protein (1-84) |
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| PKSH032854-50ug | Elabscience Biotech | 50ug | EUR 375 |
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Description: Human |
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 Human Recombinant Protein) PTH Parathyroid Hormone (7-84) Human Recombinant Protein |
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| PROTP01270-1 | BosterBio | Regular: 0.5mg | EUR 818.4 |
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Description: PTH (7-84) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 76 amino acids, having an MW of 8.8kDa. ;The PTH (7-84) is purified by proprietary chromatographic techniques. |
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 Human Recombinant Protein) PTH Parathyroid Hormone (1-34) Human Recombinant Protein |
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| PROTP01270-3 | BosterBio | Regular: 0.5mg | EUR 818.4 |
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Description: Parathyroid Hormone Human Recombinant (C181H290N55O51S2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 34 amino acids and having a molecular mass of 4117.8 Dalton. ;The PTH is purified by proprietary chromatographic techniques. |
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 Human Recombinant Protein) PTH Parathyroid Hormone (1-84) Human Recombinant Protein |
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| PROTP01270-4 | BosterBio | Regular: 0.5mg | EUR 818.4 |
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Description: Parathyroid Hormone 1-84 (full length) Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 84 amino acids, having an MW of ~10kDa.;The PTH is purified by proprietary chromatographic techniques. |
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 Human Recombinant Protein) PTH Parathyroid Hormone (7-34) Human Recombinant Protein |
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| PROTQ03431 | BosterBio | Regular: 0.5mg | EUR 818.4 |
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Description: Parathyroid Hormone Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 28 amino acids and having a molecular mass of 3380 Dalton. ;The PTH is purified by proprietary chromatographic techniques. |
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, partial (Active)) Recombinant Human Parathyroid hormone protein (PTH), partial (Active) |
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| CSB-AP000021HU | Cusabio | 8495 mg | Ask for price |
Recombinant Human Parathyroid hormone protein (PTH), partial (Active) |
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| CSB-AP000031HU | Cusabio | 8496 mg | Ask for price |
) Recombinant Human Parathyroid Hormone (PTH) |
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| MBS7115159-001mg | MyBiosource | 0.01mg | EUR 260 |
Recombinant Human Parathyroid Hormone (PTH) |
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| MBS7115159-005mg | MyBiosource | 0.05mg | EUR 545 |
Recombinant Human Parathyroid Hormone (PTH) |
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| MBS7115159-5x005mg | MyBiosource | 5x0.05mg | EUR 2250 |
) Recombinant Human Parathyroid hormone (PTH) |
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| MBS9018515-02mg | MyBiosource | 0.2mg | EUR 330 |
Recombinant Human Parathyroid hormone (PTH) |
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| MBS9018515-05mg | MyBiosource | 0.5mg | EUR 630 |
Recombinant Human Parathyroid hormone (PTH) |
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| MBS9018515-1mg | MyBiosource | 1mg | EUR 920 |
Recombinant Human Parathyroid hormone (PTH) |
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| MBS9018515-5x1mg | MyBiosource | 5x1mg | EUR 4090 |
) Recombinant Human Parathyroid Hormone/PTH Protein (His Tag) |
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| PKSH033570-10ug | Elabscience Biotech | 10ug | EUR 92 |
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Description: Human |
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Recombinant Human Parathyroid Hormone/PTH Protein (His Tag) |
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| PKSH033570-50ug | Elabscience Biotech | 50ug | EUR 178 |
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Description: Human |
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) Recombinant Human Parathyroid Hormone/PTH Protein (N-His) |
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| MBS2557179-001mg | MyBiosource | 0.01mg | EUR 155 |
Recombinant Human Parathyroid Hormone/PTH Protein (N-His) |
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| MBS2557179-005mg | MyBiosource | 0.05mg | EUR 220 |
Recombinant Human Parathyroid Hormone/PTH Protein (N-His) |
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| MBS2557179-5x005mg | MyBiosource | 5x0.05mg | EUR 980 |
) Parathyroid Hormone/PTH Protein, Human, Recombinant (hFc Tag) |
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| MBS8121322-01mg | MyBiosource | 0.1mg | EUR 395 |
Parathyroid Hormone/PTH Protein, Human, Recombinant (hFc Tag) |
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| MBS8121322-1mg | MyBiosource | 1mg | EUR 1960 |
Parathyroid Hormone/PTH Protein, Human, Recombinant (hFc Tag) |
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| MBS8121322-5x1mg | MyBiosource | 5x1mg | EUR 8665 |
 Protein) Recombinant Human Parathyroid Hormone/Parathormone/PTH (1-84) Protein |
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| MBS2553052-001mg | MyBiosource | 0.01mg | EUR 180 |
Recombinant Human Parathyroid Hormone/Parathormone/PTH (1-84) Protein |
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| MBS2553052-005mg | MyBiosource | 0.05mg | EUR 380 |
Recombinant Human Parathyroid Hormone/Parathormone/PTH (1-84) Protein |
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| MBS2553052-5x005mg | MyBiosource | 5x0.05mg | EUR 1690 |
 Recombinant Human PTH 1-84/Parathyroid hormone/Parathormone Protein |
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| E40KMP1621 | EnoGene | 20ug | EUR 495 |
Recombinant Human PTH 1-84/Parathyroid hormone/Parathormone Protein |
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| RP00477 | Abclonal | 10 μg | EUR 219.6 |
) Recombinant Human PTH / parathyroid hormone Protein (aa 32-65, GST tag) |
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| MBS2545381-01mg | MyBiosource | 0.1mg | EUR 685 |
Recombinant Human PTH / parathyroid hormone Protein (aa 32-65, GST tag) |
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| MBS2545381-5x01mg | MyBiosource | 5x0.1mg | EUR 3080 |
 (Active)) Recombinant Human Parathyroid hormone (PTH) (Active) |
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| CSB-AP005621HU | Cusabio | 8718 mg | Ask for price |
 (1-84)) Parathyroid Hormone, Recombinant, Human (PTH) (1-84) |
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| MBS637586-05mg | MyBiosource | 0.5mg | EUR 775 |
Parathyroid Hormone, Recombinant, Human (PTH) (1-84) |
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| MBS637586-5x05mg | MyBiosource | 5x0.5mg | EUR 3330 |
 (1-34)) Parathyroid Hormone, Recombinant, Human (PTH) (1-34) |
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| MBS637836-01mg | MyBiosource | 0.1mg | EUR 525 |
Parathyroid Hormone, Recombinant, Human (PTH) (1-34) |
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| MBS637836-05mg | MyBiosource | 0.5mg | EUR 895 |
Parathyroid Hormone, Recombinant, Human (PTH) (1-34) |
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| MBS637836-5x05mg | MyBiosource | 5x0.5mg | EUR 3885 |
Parathyroid Hormone, Recombinant, Human (PTH) (1-84) |
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| MBS636511-01mg | MyBiosource | 0.1mg | EUR 640 |
Parathyroid Hormone, Recombinant, Human (PTH) (1-84) |
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| MBS636511-05mg | MyBiosource | 0.5mg | EUR 1060 |
Parathyroid Hormone, Recombinant, Human (PTH) (1-84) |
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| MBS636511-1mg | MyBiosource | 1mg | EUR 1515 |
Parathyroid Hormone, Recombinant, Human (PTH) (1-84) |
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| MBS636511-5x1mg | MyBiosource | 5x1mg | EUR 6670 |
) Recombinant Human Parathyroid Hormone/PTH Protein (aa 32-65, GST Tag) |
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| PKSH030707-100ug | Elabscience Biotech | 100ug | EUR 732 |
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Description: Human |
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 N15 Labeled Human Recombinant Protein) PTH Parathyroid Hormone (1-84) N15 Labeled Human Recombinant Protein |
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| PROTP01270 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: PTH (1-84) N15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 84 amino acids and having a molecular mass of 9550 Dalton labeled by the stable isotope N15.;The PTH (1-84) N15 is purified by proprietary chromatographic techniques. |
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 N15 Labeled Human Recombinant Protein) PTH Parathyroid Hormone (7-84) N15 Labeled Human Recombinant Protein |
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| PROTP01270-2 | BosterBio | Regular: 20ug | EUR 380.4 |
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Description: PTH (7-84) N15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 78 amino acids and having a molecular mass of 8900 Dalton labeled by the stable isotope N15. |
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) Recombinant Parathyroid Hormone (PTH) |
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| RPA866Bo01 | Cloud-Clone | 10ug | EUR 164 |
