4 Host, 96T, apoptosis, array, Assay, Bacteria Pig Pigeon, Bafilomycin A1, Blocking, Ch 223191, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Goat, Green, Guinea, Mip 1B, Pamabrom 100Mg, Pepstatin A, Phospho 4Ebp1, Tubastatin A

Copper Isotopes and Copper to Zinc Ratio as Possible Biomarkers for Thyroid Cancer

Thyroid cancer is the most common endocrine cancer. There is no systematic screening for such cancer, and the current challenge is to find potential biomarkers to facilitate an early diagnosis. Copper (Cu) and zinc (Zn) are essential micronutrients involved in the proper functioning of the thyroid gland, and changes in their concentrations have been observed in the development of cancer.
Previous studies have highlighted the potential 65Cu/63Cu ratio (δ65Cu) to be a cancer biomarker. This study tests its sensitivity on plasma samples (n = 46) of Algerian patients with papillary thyroid carcinoma and a set of corresponding biopsies (n = 11). The δ65Cu ratio in blood and tumor samples was determined using multi collector inductively coupled plasma-mass spectrometry (MC-ICP-MS), and their corresponding Cu and Zn plasma total concentrations using total reflection X-ray fluorescence (TXRF).
Plasma concentrations of Cu were significantly higher (1346.1 ± 328.3 vs. 1060.5 ± 216.1 μg/L, p < 0.0001), and Zn significantly lower (942.1 ± 205.2 vs. 1027.9 ± 151.4 μg/L, p < 0.05) in thyroid cancer patients as compared to healthy controls (n = 50). Accordingly, the Cu/Zn ratio was significantly different between patients and controls (1.5 ± 0.4 vs. 1.0 ± 0.3, p < 0.0001). Furthermore, the δ65Cu plasma levels of patients were significantly lower than healthy controls (p < 0.0001), whereas thyroid tumor tissues presented high δ65Cu values. These results support the hypothesis that Cu isotopes and plasma trace elements may serve as suitable biomarkers of thyroid cancer diagnosis.

Visualization of DNA replication in single chromosome by stable isotope labeling

Among the inheritance of cellular components during cell division, deoxyribonucleic acid (DNA) and its condensate (chromosome) are conventionally visualized using chemical tag-labeled nucleotide analogs. However, associated mutagenesis with nucleotide analogs in the visualization of chromosomes is cause for concern. This study investigated the efficiency of using stable isotope labels in visualizing the replicating cultured human cell-chromosomes, in the absence of analog labels, at a high spatial resolution of 100 nm.
The distinct carbon isotope ratio between sister chromatids reflected the semi-conservative replication of individual DNA strands through cell cycles and suggested the renewal of histone molecules in daughter chromosomes. Thus, this study provides a new, powerful approach to trace and visualize cellular components with stable-isotope labeling.Key Words: stable isotope, chromosome replication, semi-conservative replication, imaging, mass spectrometry.

A high temperature water vapor equilibration method to determine non-exchangeable hydrogen isotope ratios of sugar, starch, and cellulose

The analysis of the non-exchangeable hydrogen isotope ratio (δ2 Hne ) in carbohydrates is mostly limited to the structural component cellulose, while simple high-throughput methods for δ2 Hne values of non-structural carbohydrates (NSC) such as sugar and starch do not yet exist. Here we tested if the hot vapor equilibration method originally developed for cellulose is applicable for NSC, verified by comparison with the traditional nitration method.
We set up a detailed analytical protocol and applied the method to plant extracts of leaves from species with different photosynthetic pathways (i.e., C3 , C4 , CAM). δ2 Hne of commercial sugars and starch from different classes and sources, ranging from -157.8 to +6.4‰, were reproducibly analysed with a precision between 0.2 and 7.7‰. Mean δ2 Hne values of sugar are lowest in C3 (-92.0‰), intermediate in C4(-32.5‰), and highest in CAM plants (6.0‰), with NSC being 2 H-depleted compared to cellulose and sugar being generally more 2 H-enriched than starch.
Our results suggest that our method can be used in future studies to disentangle 2 H-fractionation processes, for improving mechanistic δ2 Hne models for leaf and tree-ring cellulose, and for further development of δ2 Hne in plant carbohydrates as a potential proxy for climate, hydrology, plant metabolism and physiology. This article is protected by copyright. All rights reserved.
isotope
isotope

Rapid analysis of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) isotopologues in stable isotope-resolved metabolomics (SIRM) using direct infusion nanoelectrospray ultra-high-resolution Fourier transform mass spectrometry (DI-nESI-UHR-FTMS)

S-Adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are important metabolites in the one-carbon cycle that modulates cellular methylation required for proliferation and epigenetic regulation. Their concentrations, synthesis, and turnover are difficult to determine conveniently and reliably. We have developed such a method by coupling a simple and rapid purification scheme that efficiently captures both compounds, with high sensitivity, sample throughput direct infusion nanoelectrospray ultra-high-resolution Fourier transform mass spectrometry (DI-nESI-UHR-FTMS). This method is compatible with Stable Isotope-Resolved Metabolomic (SIRM) analysis of numerous other metabolites.
The limits of detection for both SAM and SAH were <1 nM, and the linearity range was up to 1000 nM. The method was first illustrated for SAM/SAH analysis of mouse livers, and lung adenocarcinoma A549 cells. We then applied the method to track 13C1-CH3-Met incorporation into SAM and 13C6-glucose transformation into SAM and SAH via de novo synthesis. We further used the method to show the distinct effects on A549 and H1299 cells with treatment of anti-cancer methylseleninic acid (MSA), selenite, and selenomethionine, notably SAM depletion and increased SAM to SAH ratio by MSA, which implicates altered epigenetic regulation.

Novel nickel isolation procedure for a wide range of sample matrices without using dimethylglyoxime for isotope measurements using MC-ICP-MS

Nickel isotope ratios have traditionally been used as an important tracer in cosmochemistry, and recently, it has gained attention in geochemistry, biochemistry, and environmental sciences with the development of MC-ICP-MS. Purification of Ni before isotope measurement is mandatory for obtaining precise data, which has been commonly achieved with ion-exchange chromatography, employing dimethylglyoxime (DMG) as a chelating agent for Ni.
  1. However, it has been pointed out that the use of DMG can adversely affect the isotope measurement due to insufficient Ni recovery and mass bias during measurement caused by the remaining DMG. Ni isolation procedures without the usage of DMG were innovated, but they have disadvantages such as the necessity of complex separation methods, high Ni blank, and matrix-dependent Ni recovery.
  2. Here, we present a simple Ni isolation procedure without using DMG but with the aid of oxalic acid along with common inorganic acids, achieving near-complete recovery of Ni with low blanks [0.7 ± 0.3 ng (2SD, n = 4)] only using three ion exchange column steps.
  3. To validate our method and strengthen the Ni isotope database of reference materials, 60Ni/58Ni of 20 geological reference materials, covering wide matrix compositions, were measured by MC-ICP-MS using the double-spike method.
  4. The results have shown that high recovery of Ni, independent of the sample matrix elements was achieved (98 ± 4%) and the 60Ni/58Ni was measured with a 2SD of 0.006-0.084‰ from samples containing 100-200 ng Ni.

Human Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-Hu-48T 48T
EUR 479
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Human Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-Hu-96T 96T
EUR 621
Description: A competitive inhibition quantitative ELISA assay kit for detection of Human Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Human Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-Hu-48Tests 48 Tests
EUR 500

Human Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-Hu-96Tests 96 Tests
EUR 692

Human Parathyroid Hormone (PTH) ELISA Kit, 96 tests, Quantitative

0320-PTH 1 kit
EUR 773

Bovine Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-b-48Tests 48 Tests
EUR 555

Bovine Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-b-96Tests 96 Tests
EUR 771

Canine Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-c-48Tests 48 Tests
EUR 533

Canine Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-c-96Tests 96 Tests
EUR 740

Mouse Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-Mu-48Tests 48 Tests
EUR 489

Mouse Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-Mu-96Tests 96 Tests
EUR 677

Porcine Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-p-48Tests 48 Tests
EUR 555

Porcine Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-p-96Tests 96 Tests
EUR 771

Rat Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-Ra-48Tests 48 Tests
EUR 511

Rat Parathyroid Hormone (PTH) ELISA Kit

RD-PTH-Ra-96Tests 96 Tests
EUR 709

Bovine Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-b-48T 48T
EUR 547
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-b-96T 96T
EUR 715
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-c-48T 48T
EUR 527
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Canine Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-c-96T 96T
EUR 688
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Mouse Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-Mu-48T 48T
EUR 489
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Mouse Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-Mu-96T 96T
EUR 635
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Porcine Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-p-48T 48T
EUR 547
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-p-96T 96T
EUR 715
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-Ra-48T 48T
EUR 508
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Parathyroid Hormone (PTH) ELISA Kit

DLR-PTH-Ra-96T 96T
EUR 661
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-b-48Tests 48 Tests
EUR 580

Bovine Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-b-96Tests 96 Tests
EUR 807

Canine Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-c-48Tests 48 Tests
EUR 557

Canine Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-c-96Tests 96 Tests
EUR 774

Mouse Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-Mu-48Tests 48 Tests
EUR 511

Mouse Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-Mu-96Tests 96 Tests
EUR 709

Porcine Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-p-48Tests 48 Tests
EUR 580

Porcine Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-p-96Tests 96 Tests
EUR 807

Rat Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-Ra-48Tests 48 Tests
EUR 534

Rat Parathyroid Hormone (PTH) ELISA Kit

RDR-PTH-Ra-96Tests 96 Tests
EUR 742

Recombinant Parathyroid Hormone (PTH)

4-RPA866Bo01
  • EUR 467.36
  • EUR 228.00
  • EUR 1477.60
  • EUR 559.20
  • EUR 1018.40
  • EUR 376.00
  • EUR 3544.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)

4-RPA866Ga01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Chicken Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)

4-RPA866Hu01
  • EUR 413.60
  • EUR 214.00
  • EUR 1276.00
  • EUR 492.00
  • EUR 884.00
  • EUR 340.00
  • EUR 3040.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)

4-RPA866Mu01
  • EUR 492.45
  • EUR 235.00
  • EUR 1571.68
  • EUR 590.56
  • EUR 1081.12
  • EUR 392.00
  • EUR 3779.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)

4-RPA866Po01
  • EUR 519.33
  • EUR 242.00
  • EUR 1672.48
  • EUR 624.16
  • EUR 1148.32
  • EUR 410.00
  • EUR 4031.20
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)

4-RPA866Ra01
  • EUR 476.32
  • EUR 230.00
  • EUR 1511.20
  • EUR 570.40
  • EUR 1040.80
  • EUR 382.00
  • EUR 3628.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Parathyroid Hormone expressed in: E.coli

Human Parathyroid Hormone (PTH) Protein

20-abx068425
  • EUR 578.00
  • EUR 258.00
  • EUR 1720.00
  • EUR 690.00
  • EUR 425.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Pig Parathyroid hormone (PTH)

1-CSB-MP018987PI
  • EUR 293.00
  • EUR 963.00
  • EUR 409.00
  • EUR 717.00
  • 100ug
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Pig Parathyroid hormone(PTH) expressed in Mammalian cell

Active Parathyroid Hormone (PTH)

4-APA866Ra01
  • EUR 548.00
  • EUR 250.00
  • EUR 1780.00
  • EUR 660.00
  • EUR 1220.00
  • EUR 430.00
  • EUR 4300.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Parathyroid Hormone expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Parathyroid Hormone (PTH) Antibody

20-abx110075
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx110076
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx114333
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

Parathyroid hormone (PTH) Antibody

20-abx323457
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx004483
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Parathyroid Hormone (PTH) Antibody

20-abx104853
  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx101795
  • EUR 398.00
  • EUR 133.00
  • EUR 1107.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx101796
  • EUR 411.00
  • EUR 133.00
  • EUR 1135.00
  • EUR 551.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx101797
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx101798
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx101799
  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx131970
  • EUR 314.00
  • EUR 787.00
  • EUR 411.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx008803
  • EUR 300.00
  • EUR 439.00
  • EUR 189.00
  • 100 ul
  • 200 ul
  • 30 ul

Parathyroid Hormone (PTH) Antibody

abx011748-100ul 100 ul
EUR 411

Parathyroid Hormone (PTH) Antibody

20-abx013169
  • EUR 314.00
  • EUR 98.00
  • EUR 398.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Parathyroid Hormone (PTH) Antibody

abx021231-02mg 0.2 mg
EUR 982

Parathyroid Hormone (PTH) Antibody

abx021278-1mg 1 mg
EUR 829

Parathyroid Hormone (PTH) Antibody

abx021279-1mg 1 mg
EUR 829

Parathyroid Hormone (PTH) Antibody

abx021280-1mg 1 mg
EUR 829

Parathyroid Hormone (PTH) Antibody

20-abx210160
  • EUR 439.00
  • EUR 328.00
  • 100 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx177916
  • EUR 398.00
  • EUR 133.00
  • EUR 1094.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx177917
  • EUR 398.00
  • EUR 133.00
  • EUR 1094.00
  • EUR 537.00
  • EUR 314.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx177918
  • EUR 1135.00
  • EUR 551.00
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody

20-abx177919
  • EUR 425.00
  • EUR 133.00
  • EUR 1177.00
  • EUR 578.00
  • EUR 328.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx177920
  • EUR 1233.00
  • EUR 592.00
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody

20-abx177921
  • EUR 453.00
  • EUR 133.00
  • EUR 1288.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody

20-abx173953
  • EUR 843.00
  • EUR 439.00
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody

20-abx173954
  • EUR 871.00
  • EUR 453.00
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody

20-abx173955
  • EUR 913.00
  • EUR 467.00
  • 1 mg
  • 200 ug

Parathyroid Hormone (pth) Antibody

20-abx300698
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid hormone (PTH) Antibody

abx330776-100ul 100 ul
EUR 425