4 Host, 96T, apoptosis, array, Assay, Bacteria Pig Pigeon, Bafilomycin A1, Blocking, Ch 223191, Choline Acetyltransferase Antibody, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Goat, Green, Guinea, Mip 1B, Pamabrom 100Mg, Pepstatin A, Phospho 4Ebp1, Tubastatin A

Copper Isotopes and Copper to Zinc Ratio as Possible Biomarkers for Thyroid Cancer

by Andrew Green
Thyroid cancer is the most common endocrine cancer. There is no systematic screening for such cancer, and the current challenge is to find potential biomarkers to facilitate an early diagnosis. Copper (Cu) and zinc (Zn) are essential micronutrients involved in the proper functioning of the thyroid gland, and changes in their concentrations have been observed in the development of cancer.
Previous studies have highlighted the potential 65Cu/63Cu ratio (δ65Cu) to be a cancer biomarker. This study tests its sensitivity on plasma samples (n = 46) of Algerian patients with papillary thyroid carcinoma and a set of corresponding biopsies (n = 11). The δ65Cu ratio in blood and tumor samples was determined using multi collector inductively coupled plasma-mass spectrometry (MC-ICP-MS), and their corresponding Cu and Zn plasma total concentrations using total reflection X-ray fluorescence (TXRF).
Plasma concentrations of Cu were significantly higher (1346.1 ± 328.3 vs. 1060.5 ± 216.1 μg/L, p < 0.0001), and Zn significantly lower (942.1 ± 205.2 vs. 1027.9 ± 151.4 μg/L, p < 0.05) in thyroid cancer patients as compared to healthy controls (n = 50). Accordingly, the Cu/Zn ratio was significantly different between patients and controls (1.5 ± 0.4 vs. 1.0 ± 0.3, p < 0.0001). Furthermore, the δ65Cu plasma levels of patients were significantly lower than healthy controls (p < 0.0001), whereas thyroid tumor tissues presented high δ65Cu values. These results support the hypothesis that Cu isotopes and plasma trace elements may serve as suitable biomarkers of thyroid cancer diagnosis.

Visualization of DNA replication in single chromosome by stable isotope labeling

Among the inheritance of cellular components during cell division, deoxyribonucleic acid (DNA) and its condensate (chromosome) are conventionally visualized using chemical tag-labeled nucleotide analogs. However, associated mutagenesis with nucleotide analogs in the visualization of chromosomes is cause for concern. This study investigated the efficiency of using stable isotope labels in visualizing the replicating cultured human cell-chromosomes, in the absence of analog labels, at a high spatial resolution of 100 nm.
The distinct carbon isotope ratio between sister chromatids reflected the semi-conservative replication of individual DNA strands through cell cycles and suggested the renewal of histone molecules in daughter chromosomes. Thus, this study provides a new, powerful approach to trace and visualize cellular components with stable-isotope labeling.Key Words: stable isotope, chromosome replication, semi-conservative replication, imaging, mass spectrometry.

A high temperature water vapor equilibration method to determine non-exchangeable hydrogen isotope ratios of sugar, starch, and cellulose

The analysis of the non-exchangeable hydrogen isotope ratio (δ2 Hne ) in carbohydrates is mostly limited to the structural component cellulose, while simple high-throughput methods for δ2 Hne values of non-structural carbohydrates (NSC) such as sugar and starch do not yet exist. Here we tested if the hot vapor equilibration method originally developed for cellulose is applicable for NSC, verified by comparison with the traditional nitration method.
We set up a detailed analytical protocol and applied the method to plant extracts of leaves from species with different photosynthetic pathways (i.e., C3 , C4 , CAM). δ2 Hne of commercial sugars and starch from different classes and sources, ranging from -157.8 to +6.4‰, were reproducibly analysed with a precision between 0.2 and 7.7‰. Mean δ2 Hne values of sugar are lowest in C3 (-92.0‰), intermediate in C4(-32.5‰), and highest in CAM plants (6.0‰), with NSC being 2 H-depleted compared to cellulose and sugar being generally more 2 H-enriched than starch.
Our results suggest that our method can be used in future studies to disentangle 2 H-fractionation processes, for improving mechanistic δ2 Hne models for leaf and tree-ring cellulose, and for further development of δ2 Hne in plant carbohydrates as a potential proxy for climate, hydrology, plant metabolism and physiology. This article is protected by copyright. All rights reserved.
isotope
isotope

Rapid analysis of S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) isotopologues in stable isotope-resolved metabolomics (SIRM) using direct infusion nanoelectrospray ultra-high-resolution Fourier transform mass spectrometry (DI-nESI-UHR-FTMS)

S-Adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are important metabolites in the one-carbon cycle that modulates cellular methylation required for proliferation and epigenetic regulation. Their concentrations, synthesis, and turnover are difficult to determine conveniently and reliably. We have developed such a method by coupling a simple and rapid purification scheme that efficiently captures both compounds, with high sensitivity, sample throughput direct infusion nanoelectrospray ultra-high-resolution Fourier transform mass spectrometry (DI-nESI-UHR-FTMS). This method is compatible with Stable Isotope-Resolved Metabolomic (SIRM) analysis of numerous other metabolites.
The limits of detection for both SAM and SAH were <1 nM, and the linearity range was up to 1000 nM. The method was first illustrated for SAM/SAH analysis of mouse livers, and lung adenocarcinoma A549 cells. We then applied the method to track 13C1-CH3-Met incorporation into SAM and 13C6-glucose transformation into SAM and SAH via de novo synthesis. We further used the method to show the distinct effects on A549 and H1299 cells with treatment of anti-cancer methylseleninic acid (MSA), selenite, and selenomethionine, notably SAM depletion and increased SAM to SAH ratio by MSA, which implicates altered epigenetic regulation.

Novel nickel isolation procedure for a wide range of sample matrices without using dimethylglyoxime for isotope measurements using MC-ICP-MS

Nickel isotope ratios have traditionally been used as an important tracer in cosmochemistry, and recently, it has gained attention in geochemistry, biochemistry, and environmental sciences with the development of MC-ICP-MS. Purification of Ni before isotope measurement is mandatory for obtaining precise data, which has been commonly achieved with ion-exchange chromatography, employing dimethylglyoxime (DMG) as a chelating agent for Ni.
  1. However, it has been pointed out that the use of DMG can adversely affect the isotope measurement due to insufficient Ni recovery and mass bias during measurement caused by the remaining DMG. Ni isolation procedures without the usage of DMG were innovated, but they have disadvantages such as the necessity of complex separation methods, high Ni blank, and matrix-dependent Ni recovery.
  2. Here, we present a simple Ni isolation procedure without using DMG but with the aid of oxalic acid along with common inorganic acids, achieving near-complete recovery of Ni with low blanks [0.7 ± 0.3 ng (2SD, n = 4)] only using three ion exchange column steps.
  3. To validate our method and strengthen the Ni isotope database of reference materials, 60Ni/58Ni of 20 geological reference materials, covering wide matrix compositions, were measured by MC-ICP-MS using the double-spike method.
  4. The results have shown that high recovery of Ni, independent of the sample matrix elements was achieved (98 ± 4%) and the 60Ni/58Ni was measured with a 2SD of 0.006-0.084‰ from samples containing 100-200 ng Ni.

Human Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-Hu-48Tests 48 Tests
EUR 573.6

Human Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-Hu-96Tests 96 Tests
EUR 794.4

Human Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-Hu-48Tests 48 Tests
EUR 600

Human Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-Hu-96Tests 96 Tests
EUR 830.4

Human Parathyroid Hormone (PTH) ELISA Kit, 96 tests, Quantitative
0320-PTH 1 kit
EUR 927.6

Bovine Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-b-48T 48T
EUR 656.4
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-b-96T 96T
EUR 858
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Canine Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-c-48T 48T
EUR 632.4
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Canine Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-c-96T 96T
EUR 825.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Canine Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Mouse Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-Mu-48T 48T
EUR 586.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Mouse Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-Mu-96T 96T
EUR 762
Description: A competitive inhibition quantitative ELISA assay kit for detection of Mouse Parathyroid Hormone (PTH) in samples from serum, plasma or other biological fluids.

Porcine Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-p-48T 48T
EUR 656.4
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Porcine Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-p-96T 96T
EUR 858
Description: A competitive inhibition quantitative ELISA assay kit for detection of Porcine Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-Ra-48T 48T
EUR 609.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Rat Parathyroid Hormone (PTH) ELISA Kit
DLR-PTH-Ra-96T 96T
EUR 793.2
Description: A competitive inhibition quantitative ELISA assay kit for detection of Rat Parathyroid Hormone (PTH) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-b-48Tests 48 Tests
EUR 666

Bovine Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-b-96Tests 96 Tests
EUR 925.2

Canine Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-c-48Tests 48 Tests
EUR 639.6

Canine Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-c-96Tests 96 Tests
EUR 888

Mouse Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-Mu-48Tests 48 Tests
EUR 586.8

Mouse Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-Mu-96Tests 96 Tests
EUR 812.4

Porcine Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-p-48Tests 48 Tests
EUR 666

Porcine Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-p-96Tests 96 Tests
EUR 925.2

Rat Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-Ra-48Tests 48 Tests
EUR 613.2

Rat Parathyroid Hormone (PTH) ELISA Kit
RD-PTH-Ra-96Tests 96 Tests
EUR 850.8

Bovine Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-b-48Tests 48 Tests
EUR 696

Bovine Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-b-96Tests 96 Tests
EUR 968.4

Canine Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-c-48Tests 48 Tests
EUR 668.4

Canine Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-c-96Tests 96 Tests
EUR 928.8

Mouse Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-Mu-48Tests 48 Tests
EUR 613.2

Mouse Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-Mu-96Tests 96 Tests
EUR 850.8

Porcine Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-p-48Tests 48 Tests
EUR 696

Porcine Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-p-96Tests 96 Tests
EUR 968.4

Rat Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-Ra-48Tests 48 Tests
EUR 640.8

Rat Parathyroid Hormone (PTH) ELISA Kit
RDR-PTH-Ra-96Tests 96 Tests
EUR 890.4

Recombinant Parathyroid Hormone (PTH)
4-RPA866Bo01
  • EUR 560.83
  • EUR 273.60
  • EUR 1773.12
  • EUR 671.04
  • EUR 1222.08
  • EUR 451.20
  • EUR 4252.80
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Bovine Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)
4-RPA866Ga01
  • EUR 571.58
  • EUR 276.00
  • EUR 1813.44
  • EUR 684.48
  • EUR 1248.96
  • EUR 458.40
  • EUR 4353.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Chicken Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)
4-RPA866Hu01
  • EUR 496.32
  • EUR 256.80
  • EUR 1531.20
  • EUR 590.40
  • EUR 1060.80
  • EUR 408.00
  • EUR 3648.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)
4-RPA866Mu01
  • EUR 590.94
  • EUR 282.00
  • EUR 1886.02
  • EUR 708.67
  • EUR 1297.34
  • EUR 470.40
  • EUR 4535.04
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Mouse Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)
4-RPA866Po01
  • EUR 623.20
  • EUR 290.40
  • EUR 2006.98
  • EUR 748.99
  • EUR 1377.98
  • EUR 492.00
  • EUR 4837.44
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pig Parathyroid Hormone expressed in: E.coli

Recombinant Parathyroid Hormone (PTH)
4-RPA866Ra01
  • EUR 571.58
  • EUR 276.00
  • EUR 1813.44
  • EUR 684.48
  • EUR 1248.96
  • EUR 458.40
  • EUR 4353.60
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Parathyroid Hormone expressed in: E.coli

Human Parathyroid Hormone (PTH) Protein
20-abx068425
  • EUR 693.60
  • EUR 309.60
  • EUR 2064.00
  • EUR 828.00
  • EUR 510.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx101795
  • EUR 477.60
  • EUR 159.60
  • EUR 1328.40
  • EUR 644.40
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx101796
  • EUR 493.20
  • EUR 159.60
  • EUR 1362.00
  • EUR 661.20
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx101797
  • EUR 510.00
  • EUR 159.60
  • EUR 1412.40
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx101798
  • EUR 510.00
  • EUR 159.60
  • EUR 1412.40
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx101799
  • EUR 543.60
  • EUR 159.60
  • EUR 1545.60
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx104853
  • EUR 543.60
  • EUR 159.60
  • EUR 1545.60
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx004483
  • EUR 493.20
  • EUR 710.40
  • EUR 218.40
  • EUR 376.80
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

Parathyroid Hormone (PTH) Antibody
20-abx008803
  • EUR 360.00
  • EUR 526.80
  • EUR 226.80
  • 100 ul
  • 200 ul
  • 30 ul

Parathyroid Hormone (PTH) Antibody
20-abx110075
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx110076
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx131970
  • EUR 376.80
  • EUR 944.40
  • EUR 493.20
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx114333
  • EUR 878.40
  • EUR 477.60
  • 150 ul
  • 50 ul

Parathyroid Hormone (PTH) Antibody
abx011748-100ul 100 ul
EUR 493.2

Parathyroid Hormone (PTH) Antibody
20-abx013169
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Parathyroid Hormone (PTH) Antibody
abx021231-02mg 0.2 mg
EUR 1178.4

Parathyroid Hormone (PTH) Antibody
abx021278-1mg 1 mg
EUR 994.8

Parathyroid Hormone (PTH) Antibody
abx021279-1mg 1 mg
EUR 994.8

Parathyroid Hormone (PTH) Antibody
abx021280-1mg 1 mg
EUR 994.8

Parathyroid Hormone (PTH) Antibody
20-abx210160
  • EUR 526.80
  • EUR 393.60
  • 100 ug
  • 50 ug

Parathyroid hormone (PTH) Antibody
abx330776-100ul 100 ul
EUR 510

Parathyroid Hormone (pth) Antibody
20-abx300698
  • EUR 493.20
  • EUR 2214.00
  • EUR 718.80
  • EUR 218.40
  • EUR 360.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Parathyroid hormone (PTH) Antibody
20-abx323457
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx173953
  • EUR 1011.60
  • EUR 526.80
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody
20-abx173954
  • EUR 1045.20
  • EUR 543.60
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody
20-abx173955
  • EUR 1095.60
  • EUR 560.40
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody
20-abx177916
  • EUR 477.60
  • EUR 159.60
  • EUR 1312.80
  • EUR 644.40
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx177917
  • EUR 477.60
  • EUR 159.60
  • EUR 1312.80
  • EUR 644.40
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx177918
  • EUR 1362.00
  • EUR 661.20
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody
20-abx177919
  • EUR 510.00
  • EUR 159.60
  • EUR 1412.40
  • EUR 693.60
  • EUR 393.60
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Parathyroid Hormone (PTH) Antibody
20-abx177920
  • EUR 1479.60
  • EUR 710.40
  • 1 mg
  • 200 ug

Parathyroid Hormone (PTH) Antibody
20-abx177921
  • EUR 543.60
  • EUR 159.60
  • EUR 1545.60
  • EUR 744.00
  • EUR 410.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Active Parathyroid Hormone (PTH)
4-APA866Ra01
  • EUR 657.60
  • EUR 300.00
  • EUR 2136.00
  • EUR 792.00
  • EUR 1464.00
  • EUR 516.00
  • EUR 5160.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Parathyroid Hormone expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells

Pig Parathyroid hormone (PTH)
1-CSB-MP018987PI
  • EUR 351.60
  • EUR 1155.60
  • EUR 490.80
  • EUR 860.40
  • 100ug
  • 1MG
  • 200ug
  • 500ug
Description: Recombinant Pig Parathyroid hormone(PTH) expressed in Mammalian cell