4 Host, 96T, apoptosis, array, Assay, Bacteria Pig Pigeon, Bafilomycin A1, Deoxycholic Acid Sodium Salt, Glycodeoxycholic Acid, GMO, Goat, Guinea, Mip 1B, Pepstatin A, Phospho 4Ebp1, Plate, Tubastatin A, Valproic Acid Sodium Salt

Assessing water sources for Populus simonii with different degrees of degradation based on stable isotopes

Water availability is the important thing issue limiting plant development in arid areas. Populus simonii is a typical shelterbelt tree species in Zhangbei County, Hebei Province, with an necessary function in developing ecological barrier. With steady isotope approach, graphical methodology, and a number of linear mixing mannequin, we analyzed water sources and water use methods of P. simonii in numerous development durations with 4 totally different levels of degradation (non-degraded, barely degraded, modera-tely degraded and severely degraded) in Zhangbei County.
  • Outcomes would assist enhance our understanding on the trigger and mechanism of the large-scale degradation of P. simonii on this space. The outcomes confirmed that water sources of P. simonii within the early development stage (Could-June) from all 4 degradation levels had been comparatively easy.
  • P. simonii primarily used soil water in 0-40 cm, with the utilization charges being 34.2%, 50.1%, 41.6%, and 55.7% for the 4 degradation levels, respectively. On the center development stage (July-August), non-degraded P. simonii utilized soil water from layers of 200-280 cm and 280-400 cm, with utilization charges of 20.2% and 30.9%, respectively.
  • Soil water at 200-280 cm and 280-400 cm layers was utilized by barely degraded poplar, with the contribution charges of every layer being 33.2% and 27.9%, respectively. Reasonably degraded P. simonii utilized soil water from the depths of 0-40 cm and 40-120 cm, with the charges of 30% and 26.9%, respectively. Water utilization charge of severely degraded P. simonii to 0-40 cm depth was 55.4%.
  • On the late development stage (September-October), water sources of non-degraded P. simonii transferred to the upper-middle soil layers, with the utilization charge of 0-40 cm, 40-80 cm, and 80-120 cm being 23.3%, 17.2%, and 16.5%, respectively.
  • The utilization charge of the marginally degraded P. simonii was 35.7% at 0-40 cm and 20.6% at 80-200 cm. The reasonably and severely degraded P. simonii primarily utilized soil water at 0-40 cm layer, with the contribution charges of soil water being 43.7% and 51.8%, respectively. With the exacerbation of degradation, the primary water supply of P. simonii progressively transferred from deep to floor soil water.

 

Catalytic Extremely Regioselective C-H Oxygenation Utilizing Water because the Oxygen Supply: Preparation of 17 O/ 18 O-Isotope-Labeled Compounds

We discovered that the oxygen atom of water is activated to iodosylbenzene derivatives through reversible hydrolysis of PhI(OOCR)2 and can be utilized to the oxygen supply for ruthenium(bpga)-catalyzed site-selective C-H oxygenation. Ru(bpga)/PhI(OOCR)2/H2O system, sterically much less cumbersome methinic and methylenic C-H bonds in numerous compounds could be transformed to desired oxygen practical teams in a site-selective method. Utilizing this methodology, oxygen-isotope labeled compounds akin to d-[3-17O/18O]-mannose could be ready in a multigram scale.

Partitioning ecosystem respiration of a Platycladus orientalis forest within the west mountainous space of Beijing, China utilizing steady carbon isotope

 

 

Based mostly on steady carbon isotope, we quantitatively partitioned ecosystem respiration in a Platycladus orientalis forest within the west mountainous space of Beijing. Outcomes from this examine may lay the muse for carbon change analysis in forest ecosystems of this area. The spectroscopy approach was used to constantly measure CO2 concentrations and δ13C values at totally different top of the forest. Soil and department chambers had been used for measuring nighttime δ13C values in underground and aboveground respiration, after which the proportions of respiration parts had been calculated.
Mixed with soil respiration efflux measurement, ecosystem respiration was then quantitatively partitioned. The outcomes confirmed that δ13C values of respiratory parts fluctuated, which ranged from -31.74‰ to -23.33‰ in aboveground respiration of crops and from -32.11‰ to -27.74‰ in soil respiration. The δ13C values of ecosystem respiration was on the center of these ranges.
Soil respiration averaged 1.70 μmol·m-2·s-1 at night time, accounting for 47%-91% of ecosystem respiration. Aboveground respiration averaged 0.72 μmol·m-2·s-1, contributing much less to ecosystem respiration. Daytime respiration based mostly on isotope mixing mannequin calculation had higher variability than that based mostly on temperature response mannequin, with a imply worth of two.31 μmol·m-2·s-1 and a couple of.28 μmol·m-2·s-1, respectively.
isotope
isotope

Utilizing Steady Isotope Probing and Raman Microspectroscopy to measure development charges of heterotrophic micro organism

The suitability of steady isotope probing (SIP) and Raman microspectroscopy to measure development charges of heterotrophic micro organism on the single-cell degree was evaluated. Label assimilation into E. coli biomass throughout development on a posh 13C-labeled carbon supply was monitored in time course experiments. 13C-incorporation into numerous biomolecules was measured by spectral “purple shifts” of Raman-scattered emissions.
The 13C- and 12C-isotopologues of the amino acid phenylalanine (Phe) proved to be a quantitatively correct reporter molecules of mobile isotopic fractional abundances (fcell). Values of fcell decided by Raman microspectroscopy and independently by isotope-ratio mass spectrometry (IRMS) over a spread of isotopic enrichments had been statistically indistinguishable.
Progressive labeling of Phe in E. coli cells amongst a spread of 13C/12C natural substrate admixtures occurred predictably by means of time. Relative isotopologue abundances of Phe decided by Raman spectral evaluation enabled correct calculation of bacterial development charges as confirmed independently by optical density (OD) measurements. Outcomes show that combining steady isotope probing (SIP) and Raman microspectroscopy generally is a highly effective device for learning bacterial development on the single-cell degree when grown on outlined or complicated natural 13C-carbon sources even in combined microbial assemblages.
Significance: Inhabitants development dynamics and particular person cell development charges are the last word expressions of a microorganism’s health to its environmental situations, whether or not pure or engineered. Pure habitats and plenty of industrial settings harbor complicated microbial assemblages.
Their heterogeneity in development responses to present and altering situations is commonly troublesome to know by customary methodologies. On this proof of idea examine, we examined whether or not Raman microspectroscopy can reliably quantify assimilation of isotopically-labeled vitamins into E. coli cells and allow willpower of particular person development charges amongst heterotrophic micro organism.
Raman-derived development charge estimates had been statistically indistinguishable from these derived by customary optical density measurements of the identical cultures. Raman microspectroscopy additionally could be mixed with strategies for phylogenetic identification. We report growth of Raman-based methods that allow researchers to immediately hyperlink genetic identification to practical traits and charge measurements of single cells inside combined microbial assemblages, at the moment a significant technical problem in microbiological analysis.

MCF-7-LUC cells

S0006002 One Frozen vial
EUR 543

MCF-7 Nuclear Extract

X12002 1000 µg Ask for price
Description: The best epigenetics products

MCF 7 Membrane Lysate

XBL-10442 0.1 mg
EUR 516.5
Description: MCF 7 (Human breast Adenocarcinima) cell membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The MCF 7 (Human breast Adenocarcinima) cell was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated MCF 7 (Human breast Adenocarcinima) cell membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Genomic DNA from Human Tumor Cell Line: MCF 7

D1255830 100 ug
EUR 243
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total Protein from Human Tumor Cell Line: MCF 7

P1255830 1 mg
EUR 214
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Membrane Protein from Human Tumor Cell Line: MCF 7

P3255830 0.1 mg
EUR 285
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Total RNA from Human Tumor Cell Line: MCF 7

R1255830-50 50 ug
EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Paraffin Tissue Section - Human Tumor Cell Line: MCF-7

T2255830 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

MCF-7 Nuclear Extract (H2O2)

1642-100
EUR 207

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

Human MCF-7 (breast cancer) Cell Nuclear Extract

HCL-2016 100ug
EUR 213

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-100 100 ug
EUR 164

MCF-7 Whole Cell Lysate (Human breast adenocarcinoma cells)

MCF7-50 50 ug
EUR 128

MCF-7 Human breast cancer, noninvasive cell line: >1x10^10 frozen exosomes

EXOP-100A-1 50 ug
EUR 467

GFP Expressing Human Glioblastoma Cells

TR01-GFP 500,000 Cells
EUR 1354

MCF-10A

C0006015 One Frozen vial
EUR 467

Human MCF-7 (breast cancer) Whole Cell Lysate, Hydrogen Peroxide Stimulated

HCL-2014 100ug
EUR 213

GFP Expressing Human Gastric Carcinoma N87 Cells

TR02-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Renal Adenocarcinoma Cells (ACHN)

TR04-GFP 500,000 Cells
EUR 1354

GFP Expressing Human Prostate Carcinoma Cells (DU 145)

TR03-GFP 500,000 Cells
EUR 1354

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

IGHD1-7 3'UTR GFP Stable Cell Line

TU060550 1.0 ml
EUR 2333

IGHV3-7 3'UTR GFP Stable Cell Line

TU060641 1.0 ml
EUR 2333

IGHV3OR15-7 3'UTR GFP Stable Cell Line

TU060691 1.0 ml
EUR 2333

IGHV3OR16-7 3'UTR GFP Stable Cell Line

TU060693 1.0 ml
EUR 2333

IGKV2OR2-7 3'UTR GFP Stable Cell Line

TU060855 1.0 ml
EUR 2333

IGKV3-7 3'UTR GFP Stable Cell Line

TU060861 1.0 ml
EUR 2333

IGKV3D-7 3'UTR GFP Stable Cell Line

TU060868 1.0 ml
EUR 2333

IGLV3-7 3'UTR GFP Stable Cell Line

TU060934 1.0 ml
EUR 2333

KRTAP4-7 3'UTR GFP Stable Cell Line

TU062120 1.0 ml
EUR 1394

KRTAP5-7 3'UTR GFP Stable Cell Line

TU062132 1.0 ml
EUR 1394

KRTAP9-7 3'UTR GFP Stable Cell Line

TU062151 1.0 ml
EUR 1394

KRTAP10-7 3'UTR GFP Stable Cell Line

TU062161 1.0 ml
EUR 1394

KRTAP19-7 3'UTR GFP Stable Cell Line

TU062187 1.0 ml
EUR 1394

TRAV8-7 3'UTR GFP Stable Cell Line

TU076322 1.0 ml
EUR 2333

TRBJ2-7 3'UTR GFP Stable Cell Line

TU076380 1.0 ml
EUR 2333

TRBV5-7 3'UTR GFP Stable Cell Line

TU076394 1.0 ml
EUR 2333

TRBV6-7 3'UTR GFP Stable Cell Line

TU076402 1.0 ml
EUR 2333

TRBV7-7 3'UTR GFP Stable Cell Line

TU076411 1.0 ml
EUR 2333

RNU6-7 3'UTR GFP Stable Cell Line

TU070174 1.0 ml
EUR 2333

Krtap4-7 3'UTR GFP Stable Cell Line

TU256900 1.0 ml Ask for price

Krtap16-7 3'UTR GFP Stable Cell Line

TU160815 1.0 ml Ask for price

Krtap4-7 3'UTR GFP Stable Cell Line

TU160834 1.0 ml Ask for price

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Green Fluorescent Protein (GFP-fusion protein) ELISA Kit, 96 tests, Quantitative

800-420-GFP 1 kit
EUR 712

Anti-Cytokeratin 7

DB051RTU-7 7 ml
EUR 204
Description: rabbit monospecific clonal antibodies for ihc-p application; prediluted (ready to use)

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 345
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Recombinant Human Galectin-7

7-00442 2µg Ask for price

Recombinant Human Galectin-7

7-00443 10µg Ask for price

Recombinant Human Galectin-7

7-00444 1mg Ask for price

Recombinant Human Interleukin-7

7-00895 2µg Ask for price

Recombinant Human Interleukin-7

7-00896 10µg Ask for price

Recombinant Human Interleukin-7

7-00897 1mg Ask for price

Recombinant Mouse Interleukin-7

7-00904 2µg Ask for price

Recombinant Mouse Interleukin-7

7-00905 10µg Ask for price

Recombinant Mouse Interleukin-7

7-00906 1mg Ask for price

Recombinant Human Kallikrein-7

7-03034 2µg Ask for price

Recombinant Human Kallikrein-7

7-03035 10µg Ask for price

Recombinant Human Kallikrein-7

7-03036 100µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03478 5µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03479 20µg Ask for price

Recombinant ProMatrix Metalloproteinase-7

7-03480 1mg Ask for price

Individual Reaction Mix 7

G065-7 200 reactions
EUR 167

Recombinant Human Interleukin-7, Saccharomyces

7-00898 2µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00899 10µg Ask for price

Recombinant Human Interleukin-7, Saccharomyces

7-00900 1mg Ask for price